呼出气冷凝液中LTB4和CCL11水平与儿童哮喘分期的研究

发布时间：2018-03-05 00:08

本文选题：儿童　切入点：支气管哮喘　出处：《中国现代医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的探索呼出气冷凝液(EBC)中白三烯B4(LTB4)和嗜酸性粒细胞趋化因子(CCL11)2种标记物对儿童哮喘病情的评估作用。方法收集64例哮喘患儿。其中,急性发作期33例,慢性持续期18例,缓解期13例,选同时期8例健康儿童作对照组。采用R-Tube收集每个实验对象的EBC,酶联免疫吸附法检测每个标本中LTB4和CCL11。结果 (1)EBC中LTB4水平从高到低依次为发作组(70.89±8.31)pg/ml、持续组(35.27±6.61)pg/ml、缓解组(24.58±4.90)pg/ml、对照组(15.53±3.24)pg/ml,各组间比较,差异有统计学意义(P0.05);(2)EBC中CCL11水平最高为哮喘发作组(3.38±0.65)pg/ml,其次为持续组(2.46±0.52)pg/ml、缓解组(0.90±0.36)pg/ml和对照组(0.71±0.26)pg/ml,缓解组与对照组比较,差异无统计学意义。结论 EBC中LTB4、CCL11水平能反映哮喘气道炎症及其控制情况,对哮喘患儿病情的评估有一定价值。
[Abstract]:Objective to investigate the effect of leukotriene B4TB4) and eosinophil chemokine CCL11C (CCL11C) in the assessment of asthma in children. Methods 64 children with asthma were collected, of whom 33 were in acute attack and 18 in chronic duration. There were 13 cases in remission period. Eight healthy children of the same period were selected as control group. EBC of each sample was collected by R-Tube, and LTB4 and CCL11in each sample were detected by enzyme-linked immunosorbent assay (Elisa). The results showed that the LTB4 level of EBC in the attack group was 70.89 卤8.31 渭 g / ml, and that in the persistent group was 35.27 卤6.61 PG / ml. The control group was 15.53 卤3.24 g / ml, and the control group was 24.58 卤4.90 渭 g / ml. The highest CCL11 level was 3.38 卤0.65 g / ml in asthma attack group, followed by 2.46 卤0.52 渭 g / ml in persistent group, 0.90 卤0.36 渭 g / ml in remission group and 0.71 卤0.26 PG / ml in control group. Conclusion the level of LTB4 CCL11 in EBC can reflect the airway inflammation and its control in asthmatic children.
【作者单位】：安徽医科大学研究生院;安徽医科大学第一附属医院儿科;
【分类号】：R725.6

【相似文献】