FoxO1在新疆维吾尔族先天性马蹄内翻足的表达及其临床意义

发布时间：2018-03-04 22:19

  本文选题：先天性马蹄内翻足　切入点：FoxO　出处：《重庆医学》2017年17期 　论文类型：期刊论文

【摘要】：目的探讨FoxO1基因在新疆维吾尔族先天性马蹄内翻足(ICTEV)中的表达及FoxO1与先天性马蹄内翻足畸形程度的相关性。方法运用Western Blot方法检测新疆地区51例维吾尔族先天性马蹄内翻足患儿和25例维吾尔族健康儿童足部肌肉组织中FoxO1蛋白表达水平;应用实时荧光定量PCR测定不同畸形程度维吾尔族先天性马蹄内翻足患儿FoxO1mRNA相对表达量。结果 Western Blot检测结果显示ICTEV患儿FoxO1蛋白表达水平(0.52±0.23)低于正常儿童(1.61±0.15),两组比较差异有统计学意义(P=0.017)。实时荧光定量PCR检测显示,DimeglioⅡ、Ⅲ、Ⅳ型ICTEV的FoxO1mRNA相对表达量分别为1.02±0.24、0.67±0.15、0.24±0.19;维吾尔族ICTEV畸形程度与FoxO1mRNA相对表达量呈负相关性(P=0.039)。结论 FoxO1基因转录水平随ICTEV畸形程度的加重而逐渐降低,提示FoxO1基因可能参与了维吾尔族ICTEV的整个发病过程。
[Abstract]:Objective to investigate the expression of FoxO1 gene in congenital equinovarus equinovarus in Xinjiang Uygur and the correlation between FoxO1 and the degree of congenital equinovarus deformity. Methods Western Blot method was used to detect 51 cases of congenital equinovarus deformity of Uygur nationality in Xinjiang. The expression of FoxO1 protein in the muscle tissues of the feet of children with equinovarus and 25 healthy children of Uygur nationality; The relative expression of FoxO1mRNA in Uygur children with congenital horseshoe varus with different degrees of deformity was measured by real-time fluorescence quantitative PCR. Results the expression level of FoxO1 protein in ICTEV children was 0.52 卤0.23), which was lower than that in normal children (1.61 卤0.15). The difference was statistically significant. Real time fluorescence quantitative PCR was used to detect Dimeglio 鈪，

本文编号：1567552