DNA甲基转移酶在儿童免疫性血小板减少症中的蛋白表达

发布时间：2018-03-14 10:20

  本文选题：免疫性血小板减少症　切入点：DNA甲基转移酶　出处：《中国小儿血液与肿瘤杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的通过检测DNA甲基转移酶(DNMTs)在儿童免疫性血小板减少症(ITP)外周血中蛋白水平的表达,探讨其与儿童ITP的发病机制的关系。方法收集2015年5月-2016年5月在新乡医学院第一附属医院门诊、病房就诊和治疗的60例新诊断ITP患儿(实验组)和门诊健康体检的36例儿童(对照组)为研究对象。无菌采集其空腹外周血2 mL,采取酶联免疫吸附法检测血清中DNMT1、DNMT3a、DNMT3b的蛋白表达水平。分别分析年龄、性别、血小板计数(PLT)对血清DNMT1、DNMT3a、DNMT3b的影响,及DNMT1、DNMT3a、DNMT3b三者之间的关系。结果 (1)新诊断ITP患儿PLT为(21.3±26.1)×10~9/L,明显低于健康对照组(261.2±78.4)×10~9/L,差异有显著性(t=-17.777,P=0.000)。(2)ITP患儿DNMT1、DNMT3a、DNMT3b(ug/L)的蛋白表达水平分别为(1.43±0.96)、(0.67±0.39)、(0.53±0.39),明显低于对照组(2.05±1.24)、(1.04±0.58)、(0.73±0.45),差异有显著性(t=-2.547,-3.692,-2.341;P=0.013,0.000,0.021)。经Person相关性分析,ITP患儿血小板计数与DNMT1、DNMT3a、DNMT3b的蛋白表达水平无明显相关性(r=-0.062、0.161、0.079,P=0.636、0.220、0.549)。DNMT1与DNMT3a、DNMT3b的蛋白表达无相关性(r=-0.025,-0.057;P=0.851、0.665),DNMT3a、3b的蛋白表达正相关性(r=0.259,P=0.046)。结论新诊断ITP患儿外周血中DNMT1、DNMT3a、DNMT3b的蛋白表达水平显著降低,提示DNA甲基转移酶的异常表达可能与儿童ITP的发病机制相关,推测在儿童ITP患儿DNA甲基化过程中,DNMT3a、DNMT3b起着相互协调的作用。
[Abstract]:Objective to detect the expression of DNA methyltransferase (DNA) in peripheral blood of children with immune thrombocytopenia. Methods from May 2015 to May 2016, we collected the outpatient clinic of the first affiliated Hospital of Xinxiang Medical College. Sixty newly diagnosed children with ITP (experimental group) and 36 healthy children (control group) who were admitted to the ward and treated in the hospital were studied. The fasting peripheral blood samples were collected aseptically and detected by enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay). The protein expression level of DNMT1, DNMT3a, DNMT3b, DNMT3b, DNMT3b, DNMT3b and DNMT3b. The effect of sex, platelet count and PLT on serum DNMT1, DNMT3aand DNMT3b, Results: the PLT of newly diagnosed children with ITP was 21.3 卤26.1 脳 10 ~ 9 / L, which was significantly lower than that of the healthy control group (261.2 卤78.4) 脳 10 ~ (9) / L, and the difference was statistically significant (t -17.777P _ (0.000) P _ (0.000) 路2ITP). The protein expression levels of DNMT1 / DNMT3a / DNMT3bnmugL) were 1.43 卤0.966.67 卤0.39 卤0.39 / L respectively, which was significantly lower than that of the control group (2.05 卤1.2404 卤0.580.45). Person correlation Analysis: there is no significant correlation between platelet count and the protein expression of DNMT3b in DNMT1, DNMT3a1, DNMT3b and DNMT3b. Conclusion the expression of DNMT1DNMT3b and DNMT3a DNMT3b in newly diagnosed children with ITP is significantly lower than that of DNMT3a DNMT3b. Conclusion the expression level of DNMT1DNMT3a DNMT3b in newly diagnosed children with ITP is significantly lower than that of DNMT3a DNMT3b, and the positive correlation between DNMT3b and DNMT3a3b protein expression in children with DNMT3aDNMT3b is significantly lower than that of DNMT1DNMT3a DNMT3b protein expression in children with DNMT3aDNMT3b. Conclusion the expression of DNMT1DNMT3a DNMT3b protein in newly diagnosed children with ITP is significantly lower than that of DNMT3a DNMT3b. These results suggest that the abnormal expression of DNA methyltransferase may be related to the pathogenesis of ITP in children, and it is suggested that DNMT3a1 / DNMT3b may play a coordinated role in the process of DNA methylation in children with ITP.
【作者单位】： 新乡医学院第一附属医院儿科;安阳市妇幼保健院儿科;
【分类号】：R725.5
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