社区获得性肺炎患儿EB病毒及肺炎支原体感染情况分析

发布时间：2018-03-19 23:19

本文选题：儿童　切入点：社区获得性肺炎　出处：《中国病原生物学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的分析社区获得性肺炎(CAP)患儿Epstein-Barr病毒(EBV)及肺炎支原体(MP)感染的临床特点。方法选择2015年6月至2016年6月在齐齐哈尔医学院附属第三医院住院治疗的CAP患儿280例,采血,分离血清,采用酶联免疫吸附法(ELISA)检测EBV抗体,采用被动颗粒凝集法检测MP抗体,采用实时荧光定量PCR(qPCR)检测EBV-DNA、MP-DNA。结果 ELISA和被动颗粒凝集试验检测280例CAP患儿单一EBV、MP感染率及EBV+MP混合感染率分别为7.9%(22/280)、31.1%(87/280)和18.9%(53/280),以单一MP感染占多数(P0.05);不同性别患儿单一EBV、MP感染率及EBV+MP混合感染率差异无统计学意义(P0.05);按年龄统计单一EBV感染率及EBV+MP混合感染率以3月~1岁组较高(P0.05),单一MP感染率5岁~12岁组较高(P0.05)。随病程的延长,患儿血清EBV-DNA阳性率逐渐降低,MP-DNA阳性率先升高后降低,并于入院后1周达最高(48.7%)。结论 CAP患儿以单一MP感染多见,EBV感染常与MP感染同时存在,且不同年龄患儿EBV、MP感染率不同,动态监测血清EBV-DNA、MP-DNA有助于儿童CAP的早期诊断及疾病进展评估。
[Abstract]:Objective to analyze the clinical characteristics of Epstein-Barr virus (EBV) and mycoplasma pneumoniae (MPV) infection in children with community-acquired pneumonia. Methods from June 2015 to June 2016, 280 cases of CAP were collected from the third affiliated Hospital of Qiqihar Medical College. EBV antibody was detected by enzyme-linked immunosorbent assay (Elisa) and MP antibody was detected by passive particle agglutination assay. Results ELISA and passive agglutination test were used to detect the single EBV MP infection rate and the mixed EBV MP infection rate in 280 children with CAP. Results the infection rates of single EBV MP and EBV MP in 280 cases of CAP were 7.922 / 280g / 87.87 / 280 and 18.9B 53 / 280respectively, and the majority of P0.05 patients were single MP infection. There was no significant difference between the infection rate of EBV MP and the infection rate of EBV MP, but the infection rate of single EBV and EBV MP was higher than that of the group of 1 year old in March, the infection rate of single MP was higher in the group of 5 years old and 12 years old than that of the group of 12 years old. The positive rate of serum MP-DNA decreased gradually, and reached the highest level of 48.7% at one week after admission. Conclusion single MP infection is more common in children with CAP than MP infection, and the infection rate of CAP is different in different ages. Dynamic monitoring of serum EBV-DNA MP-DNA is helpful to early diagnosis and disease progression evaluation of CAP in children.
【作者单位】：齐齐哈尔医学院附属第三医院检验科;齐齐哈尔医学院附属第三医院儿科;
【分类号】：R446.6;R725.6

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