应用配对样本提高手足口病相关肠道病毒核酸检出率

发布时间：2018-03-21 02:15

本文选题：手足口病　切入点：肠道病毒　出处：《实用医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:比较肛拭和咽拭样本中手足口病(HFMD)相关肠道病毒核酸检测结果的差异,探讨提高实验室诊断率的方法。方法:采用实时荧光定量PCR方法,对拟诊HFMD患者肛拭和咽拭配对样本进行肠道病毒71型(EV71)、柯萨奇病毒A组16型(CA16)和肠道病毒通用型(EV)RNA检测,将不同样本中各病毒的检出率进行比对,分析检测单一样本的漏检情况。结果:EV71、CA16和非EV7l/非CV16型肠道病毒(non-EV71/non-CA16 EV)在肛拭/咽拭中的检出率依次是17.25%/12.18%、4.03%/3.54%、40.28%/45.32%,肛拭样本中EV71和CA16的检出率均高于咽拭,而non-EV71/non-CA16 EV的检出率则低于咽拭,差异均具有统计学意义(P0.05);与配对样本检测结果比较,单一样本的漏检率为10.33%~36.27%。结论:应用肛拭或咽拭单一样本进行HFMD相关肠道病毒核酸检测漏检率高,采集配对样本检测能显著提高检出率。
[Abstract]:Objective: to compare the results of detection of enterovirus nucleic acid in hand, foot and mouth disease (HFMD) in anal swab and pharyngeal swab. The anal swabs and pharyngeal swabs from patients with HFMD were examined for enterovirus 71 EV71, Coxsackie virus A group 16 (CA16) and enterovirus common type (HFMD) RNA. The detection rates of each virus in different samples were compared. Results the detection rate of non-EV71% non-EVCA16 and non-EV71% non-EV16 was 17.25% 12.183.54% 40.28% 45.325.The detection rate of EV71 and CA16 in rectal swab samples was higher than that of pharyngeal swabs, but the detection rate of non-EV71/non-CA16 EV was lower than that of pharyngeal swabs. Compared with matched sample, the missing rate of single sample was 10.33 and 36.27.Conclusion: the rate of missing detection of HFMD related enterovirus nucleic acid in anus swabs or pharyngeal swabs is higher than that of matched sample. The detection rate of matched samples can be improved significantly.
【作者单位】：广西壮族自治区南宁市第四人民医院广西艾滋病临床治疗中心广西医科大学附属南宁市传染病医院检验科;
【基金】：南宁市科学研究与技术开发计划项目(编号:20123109)
【分类号】：R440;R725.1

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