常染色体显性遗传高IgE综合征患儿外周血循环B淋巴细胞亚群表型分析

发布时间：2018-03-23 09:01

本文选题：高IgE综合征　切入点：STAT基因　出处：《现代免疫学》2017年02期 　论文类型：期刊论文

【摘要】：研究STAT3突变的常染色体显性遗传高lgE综合征(autosomal dominant hyper-IgE syndrome,AD-HIES)患儿外周循环B淋巴细胞亚群变化,为研究STAT3基因在B淋巴细胞分化中的作用提供理论基础。采用多色荧光流式细胞术检测8例有明确STAT3基因诊断的AD-HIES患儿及10例正常儿童外周循环B淋巴细胞分化及亚群,并利用统计学方法分析两组B淋巴细胞亚群的差异。在骨髓及外周B淋巴细胞分化过程中,与同龄对照组相比,AD-HIES患儿外周循环初始B淋巴细胞显著增高(P=0.008),而记忆性B淋巴细胞显著降低(P0.001),其差异均存在统计学意义。在记忆性B淋巴细胞相关亚群中,与同龄正常对照组相比,AD-HIES患儿外周循环未类别转化及类别转化记忆性B淋巴细胞均显著降低(P=0.002;P=0.001),其差异均存在统计学意义。以上结果说明STAT3基因在外周血B淋巴细胞分化中起着正向调控作用。
[Abstract]:Study on STAT3 mutation of autosomal dominant lgE syndrome (autosomal dominant hyper-IgE syndrome, AD-HIES) in the peripheral circulation changes of B lymphocyte subsets, provide a theoretical basis for the study of the role of STAT3 gene in B lymphocyte differentiation. By multi-color flow cytometry in 8 AD-HIES patients with clear diagnosis and STAT3 gene 10 cases of normal children's peripheral circulation and the differentiation of B lymphocyte subsets, and the use of statistical methods for analysis of B lymphocyte subsets in two groups. The bone marrow and peripheral B lymphocyte differentiation, compared with age-matched control group, AD-HIES in peripheral B lymphocytes increased significantly in the initial cycle (P=0.008), and memory B cell significantly decreased (P0.001), the differences were statistically significant. Correlation in memory B lymphocyte subsets, compared with normal control group, AD-HIES in peripheral circulation No classification transformation and class transformation memory B lymphocytes were significantly decreased (P=0.002, P=0.001), the difference was statistically significant. The above results showed that STAT3 gene played a positive role in the regulation of B lymphocyte differentiation in peripheral blood.

【作者单位】：上海交通大学医学院附属上海儿童医学中心过敏免疫科;上海交通大学医学院附属上海儿童医学中心儿科转化医学研究所;上海交通大学医学院附属上海儿童医学中心过敏免疫联合门诊;
【基金】：国家自然科学基金(81273314;81571605) 上海市教育委员会(14ZZ105) 雅培/世界健康基金会临床营养发展中心(AFINS-HOPE-2013-15)
【分类号】：R725.9

【相似文献】