江浙沪地区EV71的分子流行病学特征

发布时间：2018-03-23 12:34

  本文选题：EV　切入点：C亚型　出处：《中华医院感染学杂志》2017年19期

【摘要】：目的研究江浙沪地区EV71-VP1遗传变异特征,了解江浙沪地区EV71病毒基因突变及抗原变异规律,为EV71的预防、治疗提供帮助。方法从GenBank上下载了江浙沪地区2009-2014年共79株EV71的VP1区段序列与各亚型参考株序列,用MEGA7.0软件构建进化树,分析江浙沪地区EV71-VP1分子流行病学特征;用疫苗株FY23株做为参考株比较江浙沪地区EV71毒株的氨基酸变异情况。结果通过进化树与序列同源分析,发现79株毒株均为C4a亚型,江浙沪地区EV71毒株可分为两个大簇与数个小簇,两个大簇间没有发现明显的序列差异,江浙沪三个地区的毒株在进化树上的分布不存在明显的差异。79株毒株共存在34个氨基酸位点突变,其中与病毒致病及疫苗接种效果相关的98、145、289位点发生多株突变,7株发生了病毒与受体结合相关的E145G/Q突变,7株发生了位于病毒表面BC环内的E98K突变,5株发生了与临床症状表现相关的A289T/V突变,未发现2009-2014年有明显的EV71毒株氨基酸变异差异。结论虽然2009-2014年江浙沪地区的EV71毒株流行亚型没有改变,也不存在明显地区间的流行差异,但是病毒的基因及氨基酸突变较为多见,仍应关注该地区的病毒基因及抗原变异,以便更好的预防EV71疫情。
[Abstract]:Objective to study the genetic variation characteristics of EV71-VP1 in Jiangsu, Zhejiang and Shanghai, and to understand the rules of gene mutation and antigen variation of EV71 virus in Jiangsu, Zhejiang and Shanghai, so as to prevent EV71. Methods from 2009 to 2014, 79 EV71 strains from Jiangsu, Zhejiang and Shanghai were downloaded from the GenBank to construct the evolutionary tree of each subtype of EV71. The molecular epidemiological characteristics of EV71-VP1 in Jiangsu, Zhejiang and Shanghai were analyzed. The amino acid variation of EV71 strains in Jiangsu, Zhejiang and Shanghai regions was compared by using vaccine FY23 strain as reference strain. Results 79 strains were found to be C4a subtype by phylogenetic tree and sequence homology analysis. The EV71 strains in Jiangsu, Zhejiang and Shanghai can be divided into two large clusters and several small clusters, and there is no obvious sequence difference between the two clusters. There was no significant difference in the distribution of virus strains in evolutionary trees between Jiangsu, Zhejiang and Shanghai. There were 34 amino acid loci mutations in 79 strains. Among them, there were several mutations at the 98145289 site associated with virus pathogenicity and inoculation effect. 7 strains of E145G/Q mutation associated with virus receptor binding occurred and 5 strains of E98K mutation located in the BC loop on the surface of the virus were found to be associated with clinical practice. A289T/V mutations associated with symptoms, No significant amino acid variation of EV71 strains was found in 2009-2014. Conclusion although the epidemic subtypes of EV71 strains in Jiangsu, Zhejiang and Shanghai regions have not changed from 2009 to 2014, there are no significant differences between regions, but the gene and amino acid mutations of the virus are more frequent. Attention should still be paid to the genetic and antigenic variation of the virus in order to better prevent the outbreak of EV71.
【作者单位】： 浙江大学医学院附属第一医院检验科;浙江省临床体外诊断技术研究重点实验室;中国人民解放军第117医院检验科;
【基金】：“十二五”国家科技重大专项基金资助项目(2012ZX10004-210)
【分类号】：R440;R725.1
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本文编号：1653477