应用基因芯片技术研究青少年特发性脊柱侧弯发病机制

发布时间：2018-03-28 14:03

本文选题：青少年特发性脊柱侧弯　切入点：基因表达　出处：《中国矫形外科杂志》2017年19期

【摘要】：[目的]应用基因芯片技术研究青少年特发性脊柱侧弯(AIS)来源的骨髓间充质干细胞(BM-MSCs)成脂分化过程中差异表达基因,探究AIS的发病机制。[方法]分离培养AIS患儿及正常对照者BM-MSCs培养至第五代(P5),成脂诱导2周后利用基因芯片技术筛选差异表达基因,并利用RT-PCR技术验证芯片结果。[结果]在AIS及正常来源的BM-MSC成脂分化中共新发现229个基因存在显著差异表达。这些差异表达基因具有细胞粘附、转录调节及信号转导等多种功能。共新发现6条具有显著统计学意义的相关分子通路。[结论]AIS患儿BM-MSCs在成脂分化过程中的基因表达模式与正常对照相比存在明显差异。本研究新发现的差异表达基因及通路可能与AIS的发病相关,对其病因学研究具有重要意义,值得深入研究。
[Abstract]:[objective] to study the differentially expressed genes in adipogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) derived from adolescent idiopathic scoliosis (AIS) by using gene chip technique. To explore the pathogenesis of AIS. [methods] the BM-MSCs of children with AIS and normal controls were isolated and cultured to the fifth generation of P5, and the differentially expressed genes were screened by gene chip technique after lipogenic induction for 2 weeks. The microarray results were verified by RT-PCR technique. [results] two hundred and twenty-nine genes were found to be differentially expressed in AIS and normal BM-MSC adipogenic differentiation, and these differentially expressed genes had cell adhesion. Transcriptional regulation and signal transduction. Six related molecular pathways with statistical significance were found. [conclusion] the gene expression pattern of BM-MSCs in children with AIS is significantly higher than that of normal controls. [conclusion] the gene expression patterns of BM-MSCs in children with AIS are significantly different from those of normal controls. Differentially expressed genes and pathways may be associated with the pathogenesis of AIS. The study of its etiology is of great significance and is worthy of further study.
【作者单位】：中山大学附属第一医院脊柱外科;
【基金】：国家自然科学基金(编号:81071439) 中国博士后科学基金项目(编号:2016M592578)
【分类号】：R726.8

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