儿童急性淋巴细胞白血病p16蛋白表达及DNA含量的临床研究

发布时间：2018-03-28 15:57

  本文选题：急性淋巴细胞性白血病　切入点：p蛋白表达　出处：《南京医科大学学报(自然科学版)》2017年04期

【摘要】：目的:探讨儿童急性淋巴细胞白血病p16蛋白表达及DNA含量的临床意义。方法:急性淋巴细胞白血病患儿58例作为观察组,健康儿童58例作为对照组,采用SP免疫组化法检测骨髓液中p16蛋白的表达水平;流式细胞术检测骨髓中白血病细胞DNA的含量,比较两组研究对象的差异。结果:观察组患儿p16阳性表达率为37.93%,明显低于对照组的100.00%(P0.05);观察组患儿p16阳性表达率与年龄、性别无明显关系(P0.05),但肝肿大4 cm患儿的p16阳性表达率明显高于≥4 cm的患儿(P0.05),高白细胞计数与高危急性淋巴细胞白血病(HR-ALL)患儿的p16阳性表达率明显高于低白细胞计数与标危急性淋巴细胞白血病(SR-ALL)患儿(P0.05)。在观察组p16蛋白阴性表达患儿中DNA异倍体发生率(66.67%)明显高于阳性表达患儿中DNA异倍体发生率(40.90%,P0.05)。结论:p16蛋白在急性淋巴细胞白血病患儿的发病过程中起到重要作用,p16蛋白缺失可能与急性淋巴细胞白血病复发相关,临床高危因素与p16阴性表达者DNA异倍体发生率升高具有相关性。
[Abstract]:Objective: to investigate the clinical significance of p16 protein expression and DNA content in children with acute lymphoblastic leukemia. Methods: 58 children with acute lymphoblastic leukemia were treated as observation group and 58 healthy children as control group. The expression of p16 protein in bone marrow fluid was detected by SP immunohistochemical method, and the DNA content of leukemic cells in bone marrow was detected by flow cytometry. Results: the positive expression rate of p16 in the observation group was 37.93, which was significantly lower than that in the control group, and the positive expression rate of p16 in the observation group was higher than that in the control group. The positive expression rate of p16 in children with hepatomegaly 4 cm was significantly higher than that in children with liver enlargement 鈮，

本文编号：1677011