婴幼儿喘息性疾病外周血单个核细胞LFA-1及Mac-1检测意义的研究

发布时间：2018-04-01 14:20

本文选题：婴幼儿喘息性疾病　切入点：淋巴细胞功能相关抗原-1　出处：《郑州大学》2012年硕士论文

【摘要】：婴幼儿喘息性疾病是目前最常见的严重危害婴幼儿健康的呼吸道感染疾病,是由多种细胞及细胞组分参与形成的气道炎症反应,进而导致气道高反应的发生。目前其病因、具体发病机制尚未完全明确。近年来,随着分子生物学研究技术的不断进步,逐渐认识到粘附分子在多种免疫应答和炎症性疾病致病过程中发挥着重要作用。本实验通过检测婴幼儿喘息性疾病淋巴细胞功能相关抗原-1(Lymphocyte Function-associated Antigen-1, LFA-1即CD11a/CD18)和巨噬细胞分化抗原-1(Macrophages differentiation antigen-1, Mac-1即CD11b/CD18)在外周血单个核细胞表面的表达情况,并研究两者之间的相关性,来探讨LFA-1、Mac-1在婴幼儿喘息性疾病发病过程中的作用机制,为进一步了解婴幼儿喘息性疾病的发病机制提供相应的理论依据和新的思路。目的本实验通过流式细胞仪检测婴幼儿喘息性疾病患儿LFA-1、Mac-1在外周血单个核细胞表面的表达情况,探讨两者在婴幼儿喘息性疾病发病过程中的作用机制。材料与方法 1研究对象及分组研究对象为存在呼吸道感染同时伴有喘息症状的患儿29例,存在呼吸道感染同时不伴有喘息症状的患儿26例,正常对照组25例,共分为喘息组、非喘息组及对照组三组。纳入本实验的所有婴幼儿采血前4周均无免疫药物治疗史；喘息组排除其它引起喘息的疾病,非喘息组及对照组儿童既往无喘息病史、个人或家族过敏性疾病史以及其他变态反应性疾病史。三组婴幼儿的年龄、性别构成差异无统计学意义。 2标本采集各组婴幼儿均于入院及体检当天采静脉血2m1,经EDTA-2N a抗凝后待用。 3实验方法采用流式细胞仪在1天内检测新鲜抗凝全血的LFA-1(CDlla+/CD18+). Mac-1(CDllb+/CD18+)的表达率。 4统计学方法用SPSS17.0统计软件包进行统计学分析,所有实验数据以均数±标准差(x±s)表示,三组间的比较采用单因素方差分析,两两比较采用LSD-t方法检验,两个因素相关性分析采用(?) Pearson(?)(?)关性检验,以P0.05为差异具有统计学意义。结果 1LFA-1结果喘息组LFA-1表达率(47.46±13.94%)高于非喘息组(35.88±12.64%,P0.01)及对照组(26.62±10.41%,P0.01),非喘息组LFA-1表达率高于对照组(P0.01)。 2Mac-1结果喘息组Mac-1表达率(7.43±3.42%)高于非喘息组(4.53±3.04%,P0.01)及对照组(2.92±1.80%,P0.01),非喘息组Mac-1表达率高于对照组(P0.05)。 3相关性分析 LFA-1与Mac-1的表达率在喘息组呈正相关(r=0.403,P0.05),而LFA-1与Mac-1的表达率在非喘息组、对照组无相关性。结论 1.LFA-1和Mac-1的异常表达参与了婴幼儿喘息性疾病的发病过程。 2.LFA-1和Mac-1在婴幼儿喘息性疾病的发病过程中具有协同作用。
[Abstract]:Infant wheezing disease is the most common respiratory tract infection that seriously endangers the health of infants and young children. It is an inflammatory reaction of airway formed by many kinds of cells and cell components, which leads to the occurrence of airway hyperresponsiveness. The specific pathogenesis is not completely clear. In recent years, with the development of molecular biology research technology, It is gradually recognized that adhesion molecules play an important role in the pathogenesis of multiple immune responses and inflammatory diseases. In this study, we detected lymphocyte function associated antigen-1 Lymphocyte Function-associated antigen-1 (LFA-1, CD11a / CD18) and macrophage in infant asthmatic disease. The expression of CD11b / CD18 on the surface of peripheral blood mononuclear cells (PBMC), and the expression of CD11b / CD18, the differentiation antigen of Macrophages differentiation antigen-1, or CD11b / CD18. The relationship between LFA-1 and Mac-1 was studied to explore the mechanism of LFA-1 Mac-1 in the pathogenesis of infant wheezing disease, and to provide a theoretical basis and a new way of thinking for further understanding the pathogenesis of infant wheezing disease. Purpose. The expression of LFA-1 Mac-1 on peripheral blood mononuclear cells (PBMC) in children with wheezing diseases was detected by flow cytometry to explore the mechanism of LFA-1 Mac-1 in the pathogenesis of wheezing diseases in infants. Materials and methods. 1. Research objects and groups. The subjects were 29 children with respiratory tract infection and wheezing symptoms, 26 children with respiratory tract infection and no wheezing symptoms, and 25 normal controls, who were divided into wheezing group. No history of immunotherapy was found in all infants and infants who were enrolled in the study before taking blood samples 4 weeks before, while other diseases causing wheezing were excluded in the wheezing group, and there was no previous history of wheezing in the non-wheezing group and the control group. There was no significant difference in age and sex composition among the three groups. 2 specimen collection. Venous blood was taken from 2 ml on admission and physical examination in each group, and was treated with EDTA-2N a anticoagulant. 3 experimental method. Flow cytometry was used to detect the expression rate of LFA-1(CDlla / CD18 / Mac-1(CDllb / CD18 in fresh anticoagulant whole blood. 4 Statistical method. All the experimental data were expressed as mean 卤standard deviation x 卤s. The comparison among the three groups was conducted by single factor analysis of variance (ANOVA). The LSD-t method was used to test the comparison between the two groups, and the correlation analysis of the two factors was used to analyze the correlation between the two factors. (Pearsonian? Cynthia? ) correlation test, with P0.05 as the difference has statistical significance. Results. 1LFA-1 results. The expression rate of LFA-1 in asthmatic group (47.46 卤13.94) was higher than that in non-asthmatic group (35.88 卤12.64) and control group (26.62 卤10.41). The expression rate of LFA-1 in non-asthmatic group was higher than that in control group (P 0.01). 2Mac-1 results. The expression rate of Mac-1 in asthmatic group (7.43 卤3.42) was higher than that in non-asthmatic group (4.53 卤3.04P0.01) and control group (2.92 卤1.80P0.01). The expression rate of Mac-1 in non-asthmatic group was higher than that in control group. 3 correlation analysis. There was a positive correlation between the expression of LFA-1 and Mac-1 in wheezing group, but no correlation between LFA-1 and Mac-1 in non-asthmatic group. Conclusion. Abnormal expression of 1.LFA-1 and Mac-1 is involved in the pathogenesis of infant wheezing disease. 2.LFA-1 and Mac-1 have synergistic effect in the pathogenesis of infant wheezing disease.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.6

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