早产儿脐血中γδ T淋巴细胞功能变化特征的研究

发布时间：2018-04-02 00:20

本文选题：脐带血　切入点：γδT细胞　出处：《免疫学杂志》2017年09期

【摘要】：目的检测分析早产儿和足月儿脐带血中γδT细胞的数量、比例及相关功能变化特征,探讨早产儿人群的γδT细胞功能变化对其抵抗病毒感染的意义。方法采用流式细胞术检测脐带血单个核细胞(cord blood mononuclear cell,CBMC)中γδT细胞的数量比例;流感病毒感染刺激早产儿和足月儿CBMC 24 h,流式细胞术测定γδT细胞中细胞因子Perforin、IFN-γ和TNF-α的表达情况;采用异戊烯焦磷酸(isopentenyl pyrophosphate,IPP)联合IL-2刺激扩增CBMC中γδT细胞,比较早产儿和足月儿γδT细胞的扩增效率。结果早产儿γδT细胞在CBMC中的数量及比例较足月儿明显减少;流感病毒刺激后,早产儿和足月儿γδT细胞的Perforin、IFN-γ和TNF-α的表达都有显著升高,但早产儿倍增幅度明显不及足月儿;经IPP和IL-2联合刺激后,脐带血中γδT细胞都有所增殖,但早产儿的扩增效率明显低于足月儿。结论早产儿γδT细胞存在数量及功能的不足,这可能是造成早产儿更易患感染性疾病的重要因素之一。
[Abstract]:Objective to detect and analyze the changes of 纬未 T cells in cord blood of premature infants and term infants. To investigate the significance of 纬未 T cell function changes in premature infants against virus infection. Methods the percentage of 纬未 T cells in cord blood mononuclear cell (blood mononuclear cell blood) was detected by flow cytometry. Influenza virus infection stimulated CBMC of premature and term infants for 24 h, the expression of cytokines Perforinine IFN- 纬 and TNF- 伪 in 纬未 T cells was detected by flow cytometry, and 纬未 T cells in CBMC were amplified by isopentenyl isopentenyl phosphate IPP combined with IL-2 stimulation, and the expression of IFN- 纬 and TNF- 伪 in 纬未 T cells were detected by flow cytometry. Results the number and proportion of 纬未 T cells in CBMC of premature infants were significantly lower than those of full-term infants, and the expression of Perforintin-IFN- 纬 and TNF- 伪 in preterm and full-term infants were significantly increased after influenza virus stimulation. After IPP and IL-2 stimulation, 纬未 T cells proliferated in umbilical cord blood, but the amplification efficiency of premature infants was significantly lower than that of term infants. Conclusion there are insufficient numbers and functions of 纬未 T cells in premature infants. This may be an important factor in preterm infants' increased susceptibility to infectious diseases.
【作者单位】：成都市血液中心;四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室;
【基金】：国家自然科学基金(81471848)
【分类号】：R722.6

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