河南省哨点医院2009～2015年5岁以下腹泻儿童轮状病毒分子流行病学特征研究

发布时间：2018-04-11 20:32

本文选题：轮状病毒 + 儿童　；参考：《病毒学报》2017年04期

【摘要】：为分析2009年~2015年河南省5岁以下腹泻儿童A组、B组、C组轮状病毒的感染状况及分子流行病学特征,本研究采集了河南省两个监测哨点医院5岁以下儿童腹泻病例的粪便样本(每份3~5ml)2 317份,双抗体夹心法检测A组轮状病毒;巢式多重RT-PCR进行基因分型,二步法多重RT-PCR检测B/C组轮状病毒,同时对阳性病例流行病学资料进行统计分析。2 317份腹泻样本共检出阳性样本869份,总阳性率35.0%,混合感染率2.5%。其中A组轮状病毒746份,阳性率32.2%;B组轮状病毒44份,阳性率1.9%;C组轮状病毒79份,阳性率3.4%;混合感染样本58份,混合感染率2.5%。A组轮状病毒阳性率的季节性特征显著,监测年度存在9~11月份(秋季)和3~5月份(春季)两个较显著高峰。A组轮状病毒G分型以G1、G2、G3、G9为主;P分型以P[8]、P[4]为主;型别组合以G1P[8]、G3P[8]、G2P[4]、G9P[8]为主,还存在G13P[8]、G234P[8]、G39P[8]、G389P[6][8]混合感染型别。河南省5岁以下腹泻患儿中以A组轮状病毒感染为主,B/C组轮状病毒感染率较低,且存在与A组混合感染模式;秋季和春季为高发季节,秋季高于春季且存在混合感染病例;大部分感染病例无发热、呕吐、脱水症状,出现发热、腹泻、呕吐、脱水等临床症状的病例多以轻症为主。
[Abstract]:In order to analyze the status of rotavirus infection and molecular epidemiological characteristics of group A and group B rotavirus in children under 5 years old in Henan Province from 2009 to 2015.In this study, fecal samples of diarrhea cases of children under 5 years old in two surveillance sentinel hospitals in Henan Province were collected (317 samples per 3~5ml)2). Group A rotavirus was detected by double antibody sandwich method, and genotyping was performed by nested multiplex RT-PCR.Two-step multiplex RT-PCR was used to detect rotavirus in group B / C, and the epidemiological data of positive cases were statistically analyzed. A total of 869 positive samples were detected from 2.217 diarrhea samples, the total positive rate was 35.0 and the mixed infection rate was 2.5%.There were 746 rotaviruses in group A, 44 rotaviruses in group B, 79 rotaviruses in group C with positive rate of 1.9% and 3.4% in group C, 58 samples of mixed infection, the seasonal characteristics of positive rate of rotavirus in group A were significant.In Henan children under 5 years old, rotavirus infection rate in group A was lower than that in group B / C, and there was a mixed infection pattern with group A, with high incidence in autumn and spring, higher incidence in autumn than in spring, and mixed infection cases in autumn and spring, and the rate of rotavirus infection in group B / C was higher than that in group A.Most of the cases without fever, vomiting, dehydration, fever, diarrhea, vomiting, dehydration and other clinical symptoms are mostly mild.
【作者单位】：河南省疾病预防控制中心传染病预防控制所;郑州市疾病预防控制中心;开封市疾病预防控制中心;
【基金】：国家“十二五科技重大专项”(项目号:2013ZX10004203),题目:艾滋病和病毒性肝炎等重大传染病防治~~
【分类号】：R440;R725.1

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