TNF-a、IL-1β在Perthes病髋关节滑膜组织中的表达及意义

发布时间：2018-04-14 13:24

  本文选题：Perthes病 + 滑膜　； 参考：《南昌大学》2014年硕士论文

【摘要】：目的： 研究TNF-a和IL-1β在Perthes病髋关节滑膜组织中的表达情况，并分析TNF-a和IL-1β在Perthes病髋关节滑膜组织中的表达的相关性，初步探讨TNF-a和IL-1β在Perthes病的发生及发展过程中所起的作用。 方法： 选取55例Perthes病患者的髋关节滑膜标本，连续组织切片，同时选择因髋部骨折行手术治疗时的髋关节滑膜16例和4例因车祸伤进行髋关节离断的患儿的髋关节滑膜做为对照组，，两组均采用免疫组化法检测TNF-a和IL-1β在患儿髋关节滑膜组织中的表达情况，并作图像及统计学分析。 结果: TNF-a和IL-1β在Perthes病患者的髋关节滑膜组织中的阳性表达率明显高于对照组髋关节滑膜组织，差异有显著统计学意义，其中TNF-a（χ2=47.46,p＜0.005），IL-1β(χ2=38.64,p＜0.005,)；而且发现在不同Catterall分期的髋关节滑膜组织中TNF-a和IL-1β的表达也不同，Ⅱ期强阳性表达为主，Ⅲ期呈中等阳性表达为主，Ⅳ期呈弱阳性表达为主，其差异有统计学意义，其中TNF-a（χ2=76.09,p＜0.005），IL-1β(χ2=77.90, p＜0.005)；TNF-a和IL-1β在Perthes病髋关节滑膜组织中的表达呈正相关关系（r=0.382, p=0.004)。 结论： TNF-a和IL-1β可能参与了Perthes病发病及进展。
[Abstract]:Objective:To study the expression of TNF-a and IL-1 尾 in synovial tissue of hip joint with Perthes disease, and to analyze the correlation between TNF-a and IL-1 尾 expression in synovial tissue of Perthes disease, and to explore the role of TNF-a and IL-1 尾 in the occurrence and development of Perthes disease.Methods:The synovium of the hip joint of 55 patients with Perthes's disease was selected as the control group. The synovium of the hip joint of 16 patients with the hip fracture treated by operation and 4 children with the injury of accident were selected as the control group, and the synovium of the hip joint was taken as the control group, and the synovium of the hip joint was removed from the synovium of the hip joint.The expression of TNF-a and IL-1 尾 in synovial tissue of hip joint was detected by immunohistochemical method.Results:The positive expression rate of TNF-a and IL-1 尾 in the synovium of hip joint of Perthes disease patients was significantly higher than that of the control group, and the difference was statistically significant.TNF-a (蠂 2 + 47.46 p < 0.005) IL-1 尾 (蠂 2 + 38.64 P < 0.005) was found to have different expressions of TNF-a and IL-1 尾 in synovial tissue of hip joint at different Catterall stages, with strong positive expression in stage 鈪

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