重庆地区呼吸道感染住院患儿病毒病原学与病情严重程度相关性分析

发布时间：2018-04-15 13:20

本文选题：呼吸道感染 + 病毒　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的：了解重庆地区急性呼吸道感染住院患儿病毒病原学情况及其与病情严重程度的相关性分析。方法：前瞻性连续收集2009年6月-2011年5月期间因急性呼吸道感染在重庆儿童医院住院患儿鼻咽抽吸物(NPA)标本1745例,提取NPA中病毒核酸、逆转录,PCR法检测14种呼吸道病毒,样本阳性采用随机核酸序列测定验证,根据呼吸道感染疾病严重程度分为重症肺炎、普通肺炎和非肺炎,分析病毒与疾病严重程度的关系。应用SAS9.13及SPSS17.0统计软件分析。结果： 1.呼吸道感染住院病毒检出率：总标本1745份,检测到至少有一种病毒阳性1318例,阳性率75.5%,其中呼吸道合胞病毒(RSV)阳性率最高阳性率,达34.2%。 2.RSV和ADV均与病情重症有关,经校正相关影响因素后,以普通肺炎和非肺炎分别作为对照组,RSV与重症肺炎相关的危险度(ORs)分别为1.65和2.36,ADV为3.44和2.94。 3. HBoV与下呼吸道感染有关,与非肺炎相比,OR值在重症肺炎中为4.06,普通肺炎中为2.31。 4. RSV混合感染其他病毒并不引起肺炎病情加重。结论： 1.重庆地区急性呼吸道感染住院患儿病毒感染阳性率高。 2. RSV和ADV均与重症肺炎关系密切。 3. HBoV与下呼吸道感染有关,在肺炎患儿中检出率高。 4.本研究结果有利于评估预防儿童呼吸道感染相关疫苗的开发及应用前景。
[Abstract]:Objective:Objective: to investigate the viral etiology of hospitalized children with acute respiratory tract infection (ARI) in Chongqing and its correlation with the severity of the disease.Methods:From June 2009 to May 2011, 1745 samples of nasopharyngeal aspirates from hospitalized children with acute respiratory infection in Chongqing Children's Hospital were collected and virus nucleic acids were extracted from NPA. Fourteen respiratory viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR).The positive samples were confirmed by random nucleic acid sequencing. According to the severity of respiratory tract infection, the patients were divided into severe pneumonia, common pneumonia and non-pneumonia. The relationship between virus and disease severity was analyzed.SAS9.13 and SPSS17.0 software were used to analyze.Results:1.The detection rate of respiratory tract infection virus: there were at least one virus positive in 1318 cases, and the positive rate was 75.5. the highest positive rate of respiratory syncytial virus (RSVV) was 34.2%.Both 2.RSV and ADV were associated with severe disease. After adjusting for the related factors, the ORs of common pneumonia and non-pneumonia as control group were 1.65 and 2.36, respectively (3.44 and 2.94).3.HBoV was associated with lower respiratory tract infection. Compared with non-pneumonia, OR was 4.06 in severe pneumonia and 2.31 in common pneumonia.4.RSV co-infection with other viruses does not aggravate pneumonia.Conclusion:1.The positive rate of viral infection in hospitalized children with acute respiratory infection in Chongqing area was high.2.RSV and ADV were closely related to severe pneumonia.3.HBoV is associated with lower respiratory tract infection and has a high detection rate in children with pneumonia.4.The results of this study are helpful to evaluate the development and application of vaccines for the prevention of respiratory tract infections in children.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.6

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