HRV间接ELISA检测方法的建立及大庆市区腹泻患儿HRV感染率的调查

发布时间：2018-04-19 06:29

本文选题：人源轮状病毒(HRV) + 分离鉴定　；参考：《黑龙江八一农垦大学学报》2012年05期

【摘要】：为了监测近期大庆市儿童人源轮状病毒(HRV)的感染情况,利用已分离鉴定好的一株HRV当地流行株,对该HRV流行株进行浓缩纯化后,以纯化的HRV作为包被抗原,按照设定的间接ELISA程序进行试验,确定并优化各种检测条件,从而建立HRV间接ELISA检测方法;利用建立的方法对2011年7月—2012年3月期间大庆地区1 452例腹泻患儿中的HRV感染情况进行初步调查,并按季节、年龄、区域、性别等方面对HRV感染率的检测结果进行统计学分析。结果显示,建立的HRV间接ELISA检测方法敏感度高、特异性强、重复性好,且检测结果灵敏、准确;利用该方法检测大庆市区1 452例腹泻患儿中HRV感染率平均为39.9%,而且得出寒冷的秋冬季是HRV感染的高发期,婴幼儿为HRV感染的易感时期;此外,卫生条件、经济状况及机体免疫力等因素也与HRV感染有一定的相关性。
[Abstract]:In order to monitor the recent infection of human rotavirus (HRV) in children in Daqing City, a local epidemic strain of HRV was isolated and identified. The strain was concentrated and purified, and the purified HRV was used as the coating antigen.According to the established indirect ELISA program, the test conditions are determined and optimized, and the indirect ELISA detection method of HRV is established.A preliminary survey of HRV infection in 1 452 children with diarrhea in Daqing area from July 2011 to March 2012 was carried out by using the established method.The results of HRV infection rate were statistically analyzed in terms of sex.The results showed that the HRV indirect ELISA assay was sensitive, specific, reproducible, sensitive and accurate.The average HRV infection rate of 1 452 children with diarrhea in Daqing urban area was 39.9, and it was concluded that cold autumn and winter were the high incidence period of HRV infection, and infants were susceptible to HRV infection.Economic status and immunity were also correlated with HRV infection.
【作者单位】：黑龙江八一农垦大学生命科学技术学院;大庆医学高等专科学校;大庆油田总医院;黑龙江八一农垦大学动物科技学院;
【分类号】：R725.1

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