静脉免疫球蛋白对早产儿脐血辅助性T细胞功能的影响

发布时间：2018-04-19 08:05

本文选题：早产儿 + 脐血　；参考：《青岛大学》2012年硕士论文

【摘要】：目的探讨不同剂量静脉免疫球蛋白(intravenous immunoglobulin,IVIG)对早产儿脐血辅助性T细胞(helper T cells,Th)免疫功能的调节作用,为早产儿临床合理应用IVIG提供实验依据。方法选取本院32-34周早产儿15例作为研究对象,密度梯度离心法分离脐血单个核细胞(cord blood mononuclear cell,CBMC),分别加入不同浓度的IVIG(A组,6mg/ml；B组,9mg/ml；C组,12mg/ml),无菌培养72小时后,ELISA法检测培养上清液中IFN-γ、IL-4的水平。结果(1)CBMC分泌IFN-γ水平变化：C组CBMC分泌IFN-γ水平(32.59±26.82pg/m1)与B组(124.51±56.06pg/ml)和A组(175.68±86.82pg/ml)相比较均显著降低,差异有极显著性意义(P＜0.01),而B组与A组比较CBMC分泌IFN-γ水平差异无显著性(P＞0.05)；(2)CBMC分泌IL-4水平变化：B组CBMC分泌IL-4水平(18.01±5.72pg/ml)和C组IL-4水平(14.44±5.18pg/ml)与A组(29.04±8.27pg/ml)相比较均显著降低,差异有极显著性意义(P＜0.01),而C组与B组比较差异无显著性(P＞0.05)；(3)CBMC培养上清液IFN-γ/IL-4比值变化：C组培养上清液IFN-γ/IL-4比值(2.41±1.72)较B组(8.22±4.21)和A组(5.61±2.38)均显著降低,差异有极显著性(P＜0.01),而B组较A组明显升高,差异有显著性(PO.05)。结论IVIG可抑制早产儿脐血Thl、Th2细胞功能,且与其剂量有关。低剂量时主要抑制Th2细胞功能；较大剂量时对Th1、Th2细胞均有明显抑制,且对Thl细胞抑制更明显。
[Abstract]:Objective to investigate the effects of intravenous immunoglobulin (IVIGI) in different doses on the immune function of umbilical cord blood helper T cells (T cells) in premature infants, and to provide experimental evidence for the rational clinical application of IVIG in premature infants.Methods Fifteen premature infants aged 32-34 weeks in our hospital were selected as subjects.Cord blood mononuclear cells were isolated from cord blood mononuclear cells by density gradient centrifugation. The levels of IFN- 纬 IL-4 in culture supernatant were detected by Elisa after 72 hours of aseptic culture.Results the level of IFN- 纬 secreted by CBMC in group C was significantly lower than that in group B (124.51 卤56.06pg / ml) and group A (175.68 卤86.82pgml / ml), and the level of IFN- 纬 secreted by CBMC was 32.59 卤26.82pg / ml in group C, significantly lower than that in group B (124.51 卤56.06pg / ml) and group A (175.68 卤86.82pgml / ml).The difference was significant (P < 0.01), but there was no significant difference in the level of CBMC secreting IFN- 纬 between group B and group A (P > 0.05). The level of IL-4 secreted by CBMC in group B was 18.01 卤5.72 PG / ml, and the level of IL-4 in group C was 14.44 卤5.18 PG / ml (P > 0.05 卤8.27 PG / ml), and the level of IL-4 in group B was significantly lower than that in group A (29.04 卤8.27 PG / ml).The difference was very significant (P < 0.01), while that in group B was significantly higher than that in group A (P < 0.01), and there was significant difference between group B and group A (P < 0.05).Conclusion IVIG can inhibit the Th2 cell function in umbilical cord blood of premature infants, and it is related to the dose of Th2 cells.The function of Th2 cells was mainly inhibited at low dose, and the Th1 / Th2 cells were significantly inhibited at higher doses, and the inhibition on Thl cells was more obvious than that on Th _ (1) T _ (2) cells.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.6

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