新生儿基因过氧化物酶体增殖激活受体PPARγC161T多态性与低出生体质量的关系

发布时间：2018-04-19 20:23

本文选题：新生儿 + 低出生体质量儿　；参考：《桂林医学院》2012年硕士论文

【摘要】：目的：探讨新生儿基因过氧化物酶增殖激活受体PPARγC161T多态性对低出生体质量(早产儿、足月小于胎龄儿)的影响。方法：用横断面调查法,使用统一的调查表,对入住桂林医学院附属医院分娩的孕妇及其单胎、活胎、低出生体质量和健康对照新生儿进行调查,共得到有效样本268个母亲-新生儿对,并根据出生体质量和孕周进行性分组,分为健康对照组和低出生体质量组,其中包括早产儿组和足月小于胎龄儿组。使用多聚酶链-限制性片段长度多态性(PCR-RFLP)方法检测其外周血基因PPARγC161T单核苷酸多态性,分析不同基因型对低出生体质量的影响。结果：1.根据双变量Logistic回归分析得出低出生体质量与父母的受教育水平、职业,家庭居住环境和氛围及维生素的补充相关,相关系数分别为-0.691、-0.434、-0.343、-0.337、-0.269、0.296、0.235。2. PPARγC161T多态性CC型、CT型与TT型分布及基因频率分布在健康对照组和低出生体质量组的足月小于胎龄儿和早产儿组分别为53.33%,14.44%,21.11%、75.00%,18.18%,6.82%和76.67%、20.00%、3.33%,早产儿组和足月小于胎龄儿组无明显差异。携带C等位基因增加低出生体质量的风险,低出生体质量组足月儿、早产儿携带CC基因型风险,与正常出生体质量儿的OR值分别为1.938(95%CT:1.001-3.75, P=0.002)、2.122(95%CI:1.091-4.127, P=0.000)。结论：低出生体质量是环境和遗传因素共同作用的结果,PPARγC161T多态性与低出生体质量有关,C等位基因可能是低出生体质量的遗传易感基因。
[Abstract]:Objective: to investigate the effect of PPAR 纬 C161T polymorphism on low birth weight (premature infants, full-term infants with small gestational age). Methods: a cross-sectional survey and a unified questionnaire were used to investigate the pregnant women and their single, live, low birth weight and health control newborns in the affiliated hospital of Guilin Medical College. A total of 268 effective mother-newborn pairs were obtained and divided into healthy control group and low birth weight group according to birth weight and gestational age. Polymerase chain restriction fragment length polymorphism (PCR-RFLP) was used to detect PPAR 纬 C161T single nucleotide polymorphisms in peripheral blood, and the effects of different genotypes on low birth weight were analyzed. The result is 1: 1. The distribution and gene frequency of PPAR 纬 C161T polymorphism CC type CT and TT genotype in healthy control group and low birth weight group were 53.33 and 14.44%, respectively, and 75.00% and 76.6777.0020.003.33%, respectively. There was no significant difference between premature infants and full-term infants with less than gestational age. Carrying C allele increased the risk of low birth weight. The odds ratio of CC genotype in low birth weight group and premature infant was 1.938 卤95% CT1: 1.001-3.75, P 0.002 + 2.122% 95% CI1.091-4.127, P0.0000.000 respectively. Conclusion: low birth weight is the result of environmental and genetic factors. PPAR 纬 C161T polymorphism is associated with low birth weight.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.1

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