一例儿童手足口病分离毒株特征分析

发布时间：2018-05-05 18:45

本文选题：手足口病 + 病原特征　；参考：《中国病原生物学杂志》2017年01期

【摘要】：目的了解河北地区1例儿童手足口病病原学特征。方法采集1例手足口病患儿支气管病理组织切片并做免疫组化分析。培养病变组织标本并分离EV71。经RT-PCR扩增EV71毒株基因及其VP1区基因,经基因测序并构建进化树,分析毒株病原特征。结果患儿支气管黏膜脱落,甚至发生坏死并伴有淋巴细胞浸润,局部小血管内形成小血栓。免疫组化分析,在支气管组织细胞中检测到阳性抗原信号,且主要集中在上皮细胞的胞浆内。RT-PCR扩增,EV71基因目的片段长度约为226bp。确定EV71阳性的标本采用RT-PCR扩增EV71毒株的VP1区基因序列,目的片段大小约为839bp。基因测序及核苷酸序列比对显示,所测序列未发生突变。构建EV71-VP1区核苷酸序列进化树,本研究中的EV71毒株属于C4亚型,其与FJ606448株的同源性达99%,未发生基因突变。结论本研究分离的EV71毒株未发生变异,与北京流行毒株同属于C4亚型,同源性较高,揭示人口流动对EV71病毒株传播影响的重要性,为河北地区儿童手足口病的临床防治提供指导。
[Abstract]:Objective to investigate the etiological characteristics of hand, foot and mouth disease (HFMD) in a child in Hebei province. Methods A case of hand, foot and mouth disease was collected and analyzed by immunohistochemistry. The diseased tissue specimens were cultured and EV71 was isolated. The gene of EV71 strain and its VP1 region gene were amplified by RT-PCR. The gene sequence and the phylogenetic tree were constructed to analyze the pathogenicity of the virus strain. Results bronchial mucosa shedding, necrosis and lymphocytic infiltration, small thrombosis in local small vessels. Immunohistochemical analysis showed that the positive antigen signal was detected in bronchial tissue cells, and the length of the target fragment of EV71 gene was about 226bpP, which was mainly concentrated in the cytoplasm of epithelial cells. RT-PCR was used to amplify the VP1 region gene sequence of EV71 strain in EV71 positive samples. The target fragment size was about 839 BP. Gene sequencing and nucleotide alignment showed no mutation. The nucleotide sequence phylogenetic tree of EV71-VP1 region was constructed. The EV71 strain in this study belongs to C4 subtype, and its homology with FJ606448 strain is 99%, and no gene mutation occurred. Conclusion the EV71 strains isolated in this study were not mutated and belonged to C4 subtype with high homology, which revealed the importance of the effect of population flow on the transmission of EV71 virus strains. To provide guidance for the clinical prevention and treatment of hand, foot and mouth disease in children in Hebei area.
【作者单位】：河北医科大学第一医院;石家庄市疾病预防控制中心流行病防治所;
【分类号】：R725.1

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