HMGB1在急性肠套叠患儿血清中的表达及其临床意义

发布时间：2018-05-07 17:21

本文选题：急性肠套叠 + 小儿　；参考：《中国现代医学杂志》2017年01期

【摘要】：目的探讨高迁移率族蛋白1(HMGB1)在小儿急性肠套叠外周血中的表达及其临床意义。方法选取2010年10月-2015年6月在该院接受治疗的急性肠套叠患儿89例,取同期体检的89例健康婴幼儿作为对照组,采用酶联免疫吸附法检测受检对象外周静脉血中HMGB1的表达,同时检测两组研究对象外周血中肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)的表达水平。结果与对照组比较,急性肠套叠患儿外周血中肿瘤坏死因子(TNF-α)、白介素6(IL-6)及HMGB1水平升高(P0.05),进一步研究发现,HMGB1的表达量与TNF-α、IL-6的表达水平相关;受试者工作特征曲线表明,以12.35 ng/ml为临界值,HMGB1用于诊断急性肠套叠的敏感性为70.79%,特异性为79.78%。结论小儿急性肠套叠外周血中HMGB1的表达量升高,早期检测其表达可能对急性肠套叠的诊断具有一定意义。
[Abstract]:Objective to investigate the expression and clinical significance of high mobility group protein 1 (HMGB1) in the peripheral blood of children with acute intussusception. Methods to select 89 cases of acute intussusception in children who were treated in the hospital in June October 2010, and take 89 healthy infants as the control group for the same period of physical examination, and the peripheral blood was detected by enzyme linked immunosorbent assay (ELISA). The expression of HMGB1 in venous blood and the expression level of tumor necrosis factor alpha (TNF- alpha) and interleukin 6 (IL-6) in the peripheral blood of two groups of subjects. Results compared with the control group, the tumor necrosis factor (TNF- alpha), interleukin 6 (IL-6) and HMGB1 (P0.05) in the peripheral blood of the children with acute intussusception were increased (P0.05). Further study found the expression of HMGB1. It was related to the expression level of TNF- alpha and IL-6, and the subjects' working characteristic curve showed that the sensitivity of HMGB1 to diagnose acute intussusception was 70.79% with 12.35 ng/ml as critical value. The specificity was 79.78%. conclusion that the expression of HMGB1 in the peripheral blood of children's acute intussusception increased, and the early detection of its expression may have a certain extent for the diagnosis of acute intussusception. Significance.

【作者单位】：河南省南阳市中心医院小儿外科;武汉大学中南医院儿科;
【基金】：湖北省卫生厅课题(No:JX3B24)
【分类号】：R725.7

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