5岁以下喘息患儿哮喘预测指数与外周血单核细胞TLR4表达的相关性研究

发布时间：2018-05-08 00:13

本文选题：Toll样受体 + 白细胞介素　；参考：《重庆医学》2017年08期

【摘要】：目的探讨5岁以下喘息患儿外周血单核细胞(PBMCs)表面Toll样受体4(TLR4)及血清白细胞介素6(IL-6)的表达。方法选取该院门诊或住院患儿共224例,其中哮喘预测指数(API)阳性组116例,阴性组108例。两组患儿均在喘息症状缓解1个月后分别抽取外周血,流式细胞仪检测PBMCs TLR4阳性率,酶联免疫吸附法(ELISA)检测血清IL-6水平。结果 API阳性组患儿CD14~+单核细胞表面TLR4阳性率明显高于API阴性组[(34.9±10.0)%vs.(30.2±8.8)%];API阳性组患儿血清IL-6水平明显高于API阴性组[(46.4±15.1)ng/Lvs.(40.5±13.6)ng/L];且两组TLR4阳性率和IL-6水平均呈线性相关(P0.05)。结论 TLR4的激活诱导IL-6等细胞因子表达可能和哮喘的发生、发展有关,TLR4可能可以作为喘息发展为哮喘的预测指标。
[Abstract]:Objective to investigate the expression of Toll like receptor 4 (TLR4) and serum interleukin 6 (IL 6) on peripheral blood mononuclear cells (PBMCs) in children under 5 years of age with wheezing. Methods A total of 224 outpatients and hospitalized children were selected, 116 of whom were positive for asthma prediction index (API) and 108 were negative for asthma prediction index (API). The peripheral blood samples were collected after the relief of wheezing symptoms for one month. The positive rate of PBMCs TLR4 was detected by flow cytometry and the serum IL-6 level was detected by enzyme linked immunosorbent assay (Elisa). Results the positive rate of TLR4 on the surface of CD14 ~ monocyte in API positive group was significantly higher than that in API negative group [34.9 卤10.0)%vs.(30.2 卤8.8%] IL-6 level was significantly higher than that in API negative group [46.4 卤15.1)ng/Lvs.(40.5 卤13.6)ng/L], and there was a linear correlation between TLR4 positive rate and IL-6 level in both groups (P 0.05). Conclusion the activation of TLR4 may induce the expression of cytokines such as IL-6, which may be related to the occurrence of asthma, and the development of TLR4 may be used as a predictor of asthma.
【作者单位】：江苏省徐州市儿童医院重症医学科;
【分类号】：R725.6

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