儿童患者下呼吸道标本苛养菌阳性分离率的影响因素分析

发布时间：2018-05-14 00:37

本文选题：下呼吸道 + 苛养菌　；参考：《中国感染与化疗杂志》2017年05期

【摘要】：目的分析儿童患者下呼吸道标本苛养菌(肺炎链球菌和流感嗜血杆菌)阳性分离率的影响因素。方法选取210份下呼吸道标本,分别在采集后0.5 h、2 h、6 h、12 h、24 h接种于血平皿和巧克力平皿,研究运送时间、运送培养基和不同接种培养基对苛养菌阳性分离率的影响。结果 200份合格下呼吸道标本研究结果显示:随着标本放置时间的增加,肺炎链球菌和流感嗜血杆菌阳性分离率呈现下降趋势,除0.5 h、2 h时间段外结果差异具有统计学意义(P0.05),但0.5 h、2 h 2个时间段样本阳性率无差异;含半固体培养基的阳性分离率在不同时间段均高于其他2种运送培养基,但差异并无统计学意义(P0.05);肺炎链球菌和流感嗜血杆菌在两种商品化平皿中阳性分离率、生长情况差别不大(P0.05)。结论选择合适的运送时间、运送培养基和接种培养基对于提高苛养菌阳性分离率具有一定的意义。
[Abstract]:Objective to analyze the factors influencing the positive isolation rate of Streptococcus pneumoniae and Haemophilus influenzae in lower respiratory tract samples of children. Methods 210 samples of lower respiratory tract were collected and inoculated in blood plate and chocolate plate for 24 h at 2 h or 6 h after collection. The effects of delivery time, transport medium and different inoculation medium on the positive isolation rate of caustically-cultured bacteria were studied. Results the results showed that the positive isolation rate of Streptococcus pneumoniae and Haemophilus influenzae showed a downward trend with the increase of the sample storage time. There were significant differences in the results except 0.5 h and 2 h, but there was no difference in the positive rate of samples in 2 h, and the positive isolation rate of the semisolid medium was higher than that of the other two kinds of transport medium at different time periods. But the difference was not statistically significant (P 0.05). The positive isolation rates of Streptococcus pneumoniae and Haemophilus influenzae in the two commercial plates were not significant. Conclusion it is of significance to select the appropriate transportation time, transport medium and inoculation medium for increasing the positive isolation rate of caustic bacteria.
【作者单位】：上海市儿童医院上海交通大学附属儿童医院检验科;
【基金】：上海申康医院发展中心市级医院临床辅助科室能力建设项目(SHDC22014012) 上海市卫计委重要薄弱学科建设(2015ZB0203)
【分类号】：R446.5;R725.6

【相似文献】