蛋白质精氨酸甲基转移酶1在先天性巨结肠中的表达

发布时间：2018-05-24 10:16

  本文选题：蛋白质精氨酸甲基转移酶1 + 先天性巨结肠　； 参考：《青岛大学》2012年硕士论文

【摘要】：目的：蛋白质精氨酸甲基转移酶1(PRMT1)在细胞中精氨酸的甲基化过程中起到重要作用,在神经元细胞的生长和发育过程中有着重要意义。先天性巨结肠(HD)的病理特点为末端肠段神经节细胞的缺乏。在本研究中,将检测PRMT1在HD患儿结肠狭窄段(HD组)及正常结肠组织(对照组)中的表达情况,探讨HD发病机制。 方法：标本取自2010年9月至2011年10月间行手术治疗患儿,20例HD组标本取自狭窄肠段(无神经节细胞肠段),20例对照组标本取自正常肠段组织。40例患儿均在聊城市人民医院和聊城市第二人民医院行手术治疗。手术中采取HD患儿痉挛段及对照组患儿新鲜结肠标本,立即送至实验室,置于-86℃低温冰箱内保存,所有标本收集后进行检测。通过Western Blot实验分析肠段PRMT1蛋白表达量；通过免疫组织化学实验观察PRMT1在结肠各层组织细胞中的表达情况。 结果：HD组狭窄段肠段PRMT1蛋白含量明显减少(p0.05)。两组肠粘膜隐窝细胞PRMT1均显著表达。在对照组粘膜下层与肌间神经丛PRMT1显著表达,HD组没有PRMT1的明显表达(p0.05),这正是HD患儿的特征。 结论：结肠组织PRMT1表达量减少可能导致HD发生；PRMT1作为一种标志物来诊断HD有一定价值。
[Abstract]:Aim: protein arginine methyltransferase 1 (PRMT1) plays an important role in the methylation of arginine in cells and plays an important role in the growth and development of neuronal cells. The histopathological feature of HD in Hirschsprung's disease is the lack of ganglion cells in the terminal segment of the intestine. In this study, the expression of PRMT1 was detected in HD patients with colon stenosis and normal colon tissues (control group) to investigate the pathogenesis of HD. Methods: 20 patients with HD received surgical treatment from September 2010 to October 2011 were collected from narrow intestinal segment (20 cases without ganglion cell segment of intestine) and 20 cases from normal intestinal segment tissue. 40 cases of children in Liaocheng City were enrolled in this study. People's Hospital and Liaocheng second people's Hospital for surgical treatment. The spastic sections of HD children and the fresh colon samples of the control group were sent to the laboratory immediately and stored in the cryogenic refrigerator at -86 鈩，

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