新基因HA117编码蛋白在儿童急性白血病中的表达及其临床意义

发布时间：2018-05-24 16:27

本文选题：HA编码蛋白 + 急性白血病　；参考：《第三军医大学学报》2012年03期

【摘要】：目的探讨多药耐药新基因HA117编码蛋白在儿童急性白血病(acute leukemia,AL)患儿骨髓单个核细胞(bone marrow mononuclear cells,BMMNC)中的表达及其临床意义。方法将67例AL患儿分为初诊组、完全缓解组和未缓解组,10例ITP患儿为对照组。应用免疫组织化学法检测BMMNC中HA117编码蛋白及P-糖蛋白(P-gp)表达。结果HA117编码蛋白在AL患儿中阳性表达率从低至高为:完全缓解组、初诊组、未缓解组,对照组呈阴性表达;初诊组中强阳性(+++)表达者其首次缓解(CR1)率(16.67%)显著低于阴性表达组(94.74%)(P0.01)及中度表达组(87.50%)(P0.05);同一患儿中HA117编码蛋白表达与P-gp表达无相关性(κ=0.195,P0.05),两者均表达者CR率(30.00%)显著低于HA117+/P-gp-、HA117-/P-gp+组(83.33%)(P0.05)及HA117-/P-gp-组(94.44%)(P0.01)。结论 HA117编码蛋白的过表达与临床化疗耐药密切相关,是儿童AL预后的不利因素,且其耐药机制可能是独立的非P-gp介导的;HA117编码蛋白与P-gp两者同时高表达提示预后不良。
[Abstract]:Objective to investigate the expression and clinical significance of multidrug resistance gene HA117 coding protein in bone marrow mononuclear cells (BMMNCs) of children with acute leukemia. Methods 67 children with AL were divided into newly diagnosed group, complete remission group and non remission group (10 cases of ITP) as control group. Immunohistochemical method was used to detect the expression of HA117 coding protein and P-glycoprotein P-gp in BMMNC. Results the positive expression rate of HA117 coding protein in children with AL was from low to high: complete remission group, newly diagnosed group, non-remission group and control group. The rate of first remission CR1 was significantly lower in the high expression group (16.67%) than that in the negative expression group (94.74% P0.01) and in the moderate expression group (87.50% P 0.05), but there was no correlation between the expression of HA117 coding protein and P-gp expression in the same child (魏 0.195 P 0.05, the CR rate was 30.005%). It was lower than HA117 / P-gp-H117-P / P-gp group (83.33) and HA117-R P-gp- group (94.44%). Conclusion the overexpression of HA117 coding protein is closely related to clinical chemotherapeutic resistance and is a negative factor in prognosis of childhood AL, and its mechanism of drug resistance may be independent non-P-gp mediated overexpression of P-gp and high expression of P-gp protein, which may indicate poor prognosis.
【作者单位】：重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地;
【分类号】：R733.71

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