Th17细胞亚群在小鼠呼吸道合胞病毒感染后宿主免疫机制中的作用

发布时间：2018-06-02 04:23

本文选题：呼吸道合胞病毒 + 辅助性T淋巴细胞　；参考：《中国老年学杂志》2017年21期

【摘要】：目的探讨辅助性T淋巴细胞(Th)17在呼吸道合胞病毒(RSV)感染小鼠肺部炎症中的变化及其在RSV感染后宿主免疫机制中的作用。方法 30只5周龄雌性BALB/c小鼠随机分为实验组和对照组各15只。对照组小鼠鼻腔滴入0.1 ml DMEM维持液(含有2.5%灭活的鸡血清),实验组小鼠鼻腔滴入0.1 ml病毒液(TCID50为107.5/ml)。在处理后的第1、3、7天分别处死小鼠,取其外周血并留取小鼠脾脏和肺脏组织。采用酶联免疫吸附法(ELISA)检测小鼠外周血中白细胞介素(IL)-17A和IL-23p19的浓度。通过苏木素-伊红(HE)染色观察RSV感染后肺组织病理变化。通过流式细胞术检测小鼠脾脏淋巴细胞中Th17细胞亚群的含量变化。结果实验组小鼠在RSV病毒感染后1、3、7 d,血清IL-17A浓度较对照组明显升高(P0.05)。在RSV病毒感染的第3天和第7天,实验组小鼠血清中IL-23p19的浓度较对照组明显升高(P0.05);在RSV病毒感染后第1、3、7天,实验组小鼠脾淋巴细胞Th17亚群比例显著高于对照组(P0.05),且RSV病毒感染后第7天明显高于第1、3天(P0.05);在RSV感染后第3天小鼠肺组织出现典型间质性肺炎表现,第7天炎性细胞明显减少。实验组第3、7天小鼠肺组织病理改变评分高于对照组(P0.05)。结论在RSV感染小鼠导致的肺部炎症反应过程中,脾淋巴细胞Th17细胞亚群水平升高并且外周血血清中IL-17A的表达增加,推测Th17细胞可能参与宿主的抗病毒免疫过程。
[Abstract]:Objective to investigate the changes of helper T lymphocyte (Th) 17 in lung inflammation of mice infected with respiratory syncytial virus (RSVV) and its role in host immune mechanism after RSV infection. Methods 30 5-week old female BALB/c mice were randomly divided into experimental group and control group. In the control group, 0.1 ml DMEM maintenance solution (containing 2.5% inactivated chicken serum) was dripped into the nasal cavity of the control group, and the TCID50 was 107.5% / ml in the nasal cavity of the experimental group. The mice were killed on the 7th day after treatment, the peripheral blood was taken and the spleen and lung tissues of the mice were retained. Elisa was used to detect the concentration of interleukin (IL) -17A and IL-23p19 in peripheral blood of mice. The pathological changes of lung tissue after RSV infection were observed by hematoxylin-eosin-hehe staining. The content of Th17 subsets in spleen lymphocytes of mice was detected by flow cytometry. Results the concentration of serum IL-17A in the experimental group was significantly higher than that in the control group on the 7th day after infection with RSV virus. On the 3rd and 7th day of RSV infection, the concentration of IL-23p19 in serum of the experimental group was significantly higher than that of the control group, and the concentration of IL-23p19 in the serum of the experimental group was significantly higher than that of the control group on the 3rd and 7th day after RSV virus infection. The percentage of Th17 subsets of spleen lymphocytes in the experimental group was significantly higher than that in the control group (P 0.05), and on the 7th day after RSV infection, it was significantly higher than that on the 1st day (P 0.05), and on the third day after RSV infection, typical interstitial pneumonia appeared in the lung tissue of the mice. On the 7th day, inflammatory cells decreased significantly. The pathological score of lung tissue in the experimental group was higher than that in the control group on the 3rd day. Conclusion during the process of pulmonary inflammation induced by RSV infection, the level of Th17 cell subsets in spleen lymphocytes and the expression of IL-17A in peripheral blood of mice were increased, suggesting that Th17 cells might participate in the process of anti-virus immunity of the host.
【作者单位】：包头市中心医院检验科;包头市中心医院儿科;
【分类号】：R725.6

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