依达拉奉对高胆红素血症新生大鼠血清NSE的影响

发布时间：2018-06-06 03:01

本文选题：依达拉奉 + 神经元特异性烯醇化酶　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:探讨依达拉奉干预对高胆红素血症新生大鼠血清神经元特异性烯醇化酶(neuron-specific enolase,NSE)水平的影响。方法:将72只7日龄SD大鼠随机地分到H、E、C组,H组为高胆红素血症组(n=24)、E组为依达拉奉干预组(n=24)、C组为正常对照组(n=24)。每组又根据处死时间的不同随机分为3个亚组:12h组、24h组、48h组,每亚组8只。采用腹腔注射的方法,给H组、E组新生大鼠按100μg/g的剂量注入胆红素1次建立高胆红素血症动物模型,用同样的方法给C组新生大鼠按100μg/g的剂量注入生理盐水1次,造模后立即给予E组依达拉奉干预,剂量为2mg/kg/次,一日一次。观察各组新生大鼠有无行为异常如有无抽搐、活动减少、对外界刺激的逃避反应减弱等,并于各时间点取血及脑组织。苏木素-伊红染色(hematoxylin-eosin staining,HE)观察各组新生大鼠脑组织的病理改变,用酶联免疫法(ELISA法)测定血清中NSE。结果:1.实验前,H、E、C3组新生大鼠行为均无明显的异常;造模后H组、E组新生SD大鼠出现行为异常,如抽搐、活动减少、对外界刺激的逃避反应减弱等,但E组在行为异常方面较高胆红素血症组轻;C组新生SD大鼠在整个实验中均未出现行为方面的明显异常。2.HE染色结果:C组海马区细胞排列整齐,细胞形态结构完整,无明显细胞水肿;H组海马区细胞排列略紊乱,细胞水肿明显,有明显炎性细胞浸润;E组脑组织可见少许神经细胞水肿,但在各时间点上脑组织病理改变均较H组轻。3.在各时间点上,H组新生大鼠血清NSE水平较C组高,P0.05,差异具有统计学意义;在各时间点上,E组新生大鼠血清NSE水平较H组低,但仍高于C组,P0.05,差异有统计学意义。结论:新生大鼠高胆红素血症时,血清NSE水平增加,给予依达拉奉干预,可以降低血清NSE水平,表明依达拉奉对高胆红素血症脑损伤新生大鼠有神经保护作用。
[Abstract]:Aim: to investigate the effect of Edaravone on serum neuron specific enolase neuron-specific enolase (NSE) levels in neonatal rats with hyperbilirubinemia. Methods: Seventy-two 7-day-old SD rats were randomly divided into two groups: group H was hyperbilirubinemia group (n = 24), group E was Edaravone intervention group (n = 24), group C was normal control group (n = 24). According to the time of death, each group was randomly divided into 3 subgroups: 12 h group, 24 h group, 48 h group, 8 rats in each subgroup. The hyperbilirubinemia animal model was established by intraperitoneal injection of bilirubin at 100 渭 g / g dose to the neonatal rats in group H, and normal saline was injected to the newborn rats in group C at a dose of 100 渭 g / g. Group E was treated with Edaravone at the dose of 2mg/kg/ once a day. We observed whether the newborn rats in each group had behavioral abnormalities, such as convulsions, decreased activity, decreased escape response to external stimulation, and took blood and brain tissue at each time point. Hematoxylin-eosin staining (hematoxylin-eosin) was used to observe the pathological changes in the brain tissue of neonatal rats in each group, and the serum NSE was determined by enzyme-linked immunosorbent assay (Elisa). The result is 1: 1. Before the experiment, there was no obvious abnormality in the behavior of newborn rats in group C 3, and abnormal behavior of newborn SD rats in group E of H group after modeling, such as convulsion, decreased activity, weak escape response to external stimuli, etc. However, there was no obvious behavioral abnormality in group E with hyperbilirubinemia. 2. The results of HE staining showed that the hippocampal cells in group E were arranged neatly, and the morphology and structure of the cells were intact. There was no obvious cell edema in the hippocampal area of group H, but there were some neuronal edema in the brain tissue of group E, but the pathological changes of brain tissue in group H were lighter than those in group H at different time points. The serum NSE level in group H was significantly higher than that in group C (P 0.05), and that in group E was lower than that in group H at each time point, but still higher than that in group C (P 0.05). Conclusion: the level of serum NSE in neonatal rats with hyperbilirubinemia is increased. Edaravone intervention can decrease the level of serum NSE, indicating that Edaravone has neuroprotective effect on neonatal rats with hyperbilirubinemia brain injury.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R722.1

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