组胺H2、H4受体在过敏性紫癜炎症细胞中的表达及临床意义

发布时间：2018-06-13 12:50

本文选题：组胺H2受体 + 组胺H4受体　；参考：《第二军医大学》2014年硕士论文

【摘要】：研究目的 1、比较过敏性紫癜患儿急性期、恢复期及健康体检儿童外周血单个核细胞表面组胺H2、H4受体的表达差异,探讨组胺H2、H4受体与过敏性紫癜的关系。 2、比较过敏性紫癜非肾炎组、过敏性紫癜肾炎组组胺H2、H4受体表达的差异,了解组胺受体与过敏性紫癜肾炎的相关性。 3、比较予组胺H2受体拮抗剂西咪替丁治疗前后过敏性紫癜患儿组胺H2受体的变化,从临床的角度探讨组胺H2受体拮抗剂对过敏性紫癜组胺受体表达的影响。 4、检测过敏性紫癜患儿急性期、恢复期及健康儿童血清IL-8水平差异,了解细胞因子IL-8与过敏性紫癜的关系。 5、比较过敏性紫癜非肾炎组、肾炎组血清IL-8含量差异,了解IL-8是否与过敏性紫癜肾炎的发病相关。研究方法 1、组胺H2、H4受体在过敏性紫癜患儿炎症细胞中的表达选取来我院就诊的儿童,过敏性紫癜急性期患儿、过敏性紫癜恢复期患儿、健康体检儿童各32例,用实时定量荧光聚合酶链反应(Real-time fluorescent quantification PCR, RT-PCR)及Western蛋白印迹法(Western blot)检测三组对象外周血单个核细胞(PBMCs)中H2、H4受体的基因含量及蛋白表达。 2、过敏性紫癜急性期患儿予组胺H2受体拮抗剂西咪替丁治疗前后H2R的表达选取来我院就诊的过敏性紫癜急性期患儿,分为西咪替丁组、非西咪替丁组,用实时定量荧光聚合酶链反应(Real-time fluorescent quantification PCR, RT-PCR)及Western蛋白印迹法(Western blot)检测两组治疗前后H2R的基因含量和蛋白表达。 3、血清IL-8在过敏性紫癜中的水平采用酶联免疫吸附试验(emzyme linked immunosorbent asssy, ELISA)检测过敏性紫癜急性期患儿、过敏性紫癜恢复期患儿、健康体检儿童外周血血清IL-8的含量。结果 1、组胺H2、H4受体在过敏性紫癜中的表达过敏性紫癜急性期患儿单个核细胞中H2R的mRNA和蛋白的表达均高于过敏性紫癜恢复期儿童及健康儿童(p0.05),过敏性紫癜恢复期患儿H2R受体表达与健康儿童相比无显著差异(p0.05)。过敏性紫癜急性期组H2R的mRNA水平为(1.80±0.49),恢复期H2R的mRNA为(1.22±0.12),健康对照组水平为(1.22±0.18),过敏性紫癜急性期患儿PBMCs中H2R mRNA表达高于过敏性紫癜恢复期患儿、健康儿童(p0.05),过敏性紫癜恢复期、健康儿童之间表达量无统计学差异(p0.05)。过敏性紫癜急性期患儿单个核细胞中H4R的mRNA和蛋白的表达均高于过敏性紫癜恢复期儿童及健康儿童(p0.05),过敏性紫癜恢复期儿童H4受体表达与健康儿童相比无显著差异(p0.05)。过敏性紫癜急性期H4R的mRNA水平为(4.49±0.99),恢复期H4R的mRNA为(1.49±0.32),健康对照组H4R的mRNA水平为(1.44±0.33),过敏性紫癜急性期患儿PBMCs中H4RmRNA表达高于过敏性紫癜恢复期患儿、健康儿童(p0.05),过敏性紫癜恢复期、健康儿童之间表达量无统计学差异(p0.05) 将过敏性紫癜急性期患儿根据是否有血尿、蛋白尿等尿检异常分为非肾炎组(NHSPN组)和肾炎组(HSPN组)。非肾炎组患儿H2R的mRNA水平为(1.69±0.52),肾炎组的mRNA水平为(1.88±0.47),健康对照组H2R的mRNA水平为(1.22±0.18)。非肾炎组患儿H4R的mRNA水平为(4.59±1.34),肾炎组的H4R的mRNA水平为(4.39±0.53),健康对照组H4R的mRNA水平为(1.44±0.33)。非肾炎组、紫癜肾炎组H2R表达水平均显著高于健康对照组(p0.05),非肾炎组和肾炎组两组间H2R表达水平无明显差异(p0.05)。过敏性紫癜非肾炎组、肾炎组H4R表达水平均显著高于健康对照组(p0.05),非肾炎组和肾炎组两组间H4R表达量无显著差异(p0.05)。 2、过敏性紫癜急性期患儿予组胺H2受体拮抗剂西咪替丁治疗前后组胺H2R的表达西咪替丁组治疗前H2R的mRNA水平为(1.77±0.51),非西咪替丁组治疗前H2R的mRNA水平为(1.82±0.50),健康对照组H2R的mRNA水平为(1.22±0.18),西咪替丁组、非西咪替丁组治疗前H2R水平均高于健康对照组(p0.01)。但西咪替丁组、非西咪替丁组治疗前H2R的mRNA表达无显著性差异(p0.05),具有可参照意义。经治疗,西咪替丁组治疗后H2R的mRNA水平为(1.07±0.05),非西咪替丁组治疗后H2R的mRNA水平为(1.44±0.34),两组治疗后H2R的mRNA表达水平有显著差异(p0.05),西咪替丁治疗组H2RmRNA水平变化较大。 3、血清IL-8在过敏性紫癜中的水平过敏性紫癜急性期患儿血清中,IL-8的含量为(332.04±83.48)pg/ml,恢复期患儿血清中IL-8的含量为(97.75±18.16)pg/ml,健康对照组患儿血清中IL-8的含量为(90.9±9.73)pg/ml。过敏性紫癜急性期患儿血清中IL-8水平明显高于过敏性紫癜恢复期患儿、健康儿童(p0.05),过敏性紫癜恢复期、健康儿童之间水平量无统计学差异(p0.05)。过敏性紫癜非肾炎组患儿血清IL-8的含量为(300.86±98.62)pg/ml,肾炎组血清中IL-8的含量为(359.56±57.24)pg/ml,健康对照组IL-8水平为(90.9±9.73)pg/ml。肾炎组患儿、非肾炎组患儿血清中IL-8水平均明显高于健康对照组患儿IL-8水平。过敏性紫癜非肾炎组和肾炎组两组间IL-8含量有明显差异(p0.05),肾炎组IL-8水平高于非肾炎组。结论 1、过敏性紫癜急性期患儿单个核细胞中组胺H2R、H4R蛋白和nRNA的表达均明显高于过敏性紫癜恢复期患儿、健康儿童。经治疗症状缓解的过敏性紫癜恢复期患儿H2R、H4R的表达能降至健康儿童水平。组胺H2R, H4R可能参与过敏性紫癜发病过程。 2、过敏性紫癜急性期非肾炎组、肾炎组患儿单个核细胞中组胺H2R、H4R蛋白和mRNA的表达均高于健康儿童,过敏性紫癜非肾炎组和肾炎组两组间H2R、H4R表达水平无明显差异。过敏性紫癜是否累及肾脏,组胺H4R的表达无明显差异。 3、过敏性紫癜急性期患儿,予H2R受体拮抗剂西咪替丁治疗组治疗前后组胺H2R变化较非西咪替丁治疗组组胺H2R变化有明显差异,西咪替丁可减少组胺受体的表达。 4、过敏性紫癜急性期患儿血清IL-8含量明显高于过敏性紫癜恢复期患儿、健康儿童。经治疗症状缓解的过敏性紫癜恢复期患儿IL-8含量能降至健康儿童水平。IL-8可能参与过敏性紫癜发病过程。 5、过敏性紫癜肾炎患儿血清IL-8含量高于非肾炎组,IL-8可能参与紫癜性肾炎的发生及发展。
[Abstract]:research objective
1, to compare the expression of histamine H2 and H4 receptor on the surface of peripheral blood mononuclear cells in children with anaphylactoid purpura during the acute period, recovery and health examination, and to explore the relationship between histamine H2, H4 receptor and Henoch Schonlein purpura.
2, compare the difference of histamine H2 and H4 receptor expression between Henoch Schonlein purpura nephritis group and Henoch Schonlein purpura nephritis group, and understand the correlation between histamine receptor and Henoch Schonlein purpura nephritis.
3, the changes of histamine H2 receptor in children with Henoch Schonlein purpura before and after cimetidine treated with histamine H2 receptor antagonist were compared, and the effect of histamine H2 receptor antagonist on the expression of histamine receptor in Henoch Schonlein purpura was investigated from a clinical point of view.
4, to detect the difference of serum IL-8 levels between children with acute purpura in acute stage, convalescent stage and healthy children, and to understand the relationship between cytokine IL-8 and allergic purpura.
5, to compare the difference of serum IL-8 content between the nephritis group and the non nephritis group of Henoch Schonlein purpura, and to know whether IL-8 is related to the pathogenesis of Henoch Schonlein purpura nephritis.
research method
1, the expression of histamine H2 and H4 receptors in inflammatory cells of children with Henoch Schonlein purpura.
Children in our hospital, children with acute Henoch Schonlein purpura, children with anaphylactoid purpura, 32 children in healthy physical examination were selected, and three groups of peripheral blood mononuclear cells (PBMCs) were detected by real-time quantitative fluorescent polymerase chain reaction (Real-time fluorescent quantification PCR, RT-PCR) and Western Western blot (Western blot). Gene content and protein expression of H2, H4 receptor.
2, the expression of H2R in the acute phase of Henoch Schonlein purpura before and after treatment with histamine H2 receptor antagonist cimetidine.
Children with anaphylactoid purpura in our hospital were selected to be divided into cimetidine group, non cimetidine group, Real-time fluorescent quantification PCR (RT-PCR) and Western Western blot (Western blot) to detect the gene content and protein expression of H2R in two groups before and after treatment.
3, the level of serum IL-8 in Henoch Schonlein purpura
Emzyme linked immunosorbent asssy (ELISA) was used to detect the levels of serum IL-8 in children with anaphylactoid purpura at the acute stage of anaphylactoid purpura, in children with anaphylactoid purpura, and in healthy children.
Result
1, the expression of histamine H2, H4 receptor in Henoch Schonlein purpura
The expression of mRNA and protein of H2R in mononuclear cells in children with anaphylactoid purpura was higher than that of children with anaphylactoid purpura and healthy children (P0.05). There was no significant difference in the expression of H2R receptor in children with Henoch Schonlein purpura at the recovery period (P0.05). The mRNA level of H2R in anaphylactoid purpura acute phase group was (1.80 + 0.49), and the recovery period H2R The mRNA was (1.22 + 0.12), and the level of the healthy control group was (1.22 + 0.18). The expression of H2R mRNA in the children with anaphylactoid purpura at the acute stage of PBMCs was higher than that of the anaphylactoid purpura. There was no statistical difference between healthy children (P0.05) and the recovery period of anaphylactoid purpura. There was no statistical difference between the healthy children (P0.05).
The expression of mRNA and protein of H4R in mononuclear cells in children with anaphylactoid purpura was higher than that of children with anaphylactoid purpura and healthy children (P0.05). There was no significant difference in the expression of H4 receptor in children with anaphylactoid purpura at the recovery period (P0.05). The mRNA level of H4R in anaphylactoid purpura was (4.49 + 0.99) and H4R mR in the recovery period. NA was (1.49 + 0.32), and the level of mRNA of H4R in healthy control group was (1.44 + 0.33). The expression of H4RmRNA in children with anaphylactoid purpura at the acute stage of PBMCs was higher than that of children with anaphylactoid purpura, healthy children (P0.05), anaphylactoid purpura recovery period, and no difference in expression between healthy children (P0.05).
The patients with anaphylactoid purpura at acute stage were divided into non nephritis group (group NHSPN) and nephritis group (group HSPN). The mRNA level of H2R in the non nephritis group was (1.69 + 0.52), the mRNA level of the nephritis group was (1.88 + 0.47), and the mRNA level of H2R in the healthy control group was (1.22 + 0.18). The mRNA level of H4R in the non nephritis group was in the control group. The mRNA level of H4R in nephritis group was (4.39 + 0.53), and the mRNA level of H4R in the healthy control group was (1.44 + 0.33). The H2R expression level of the non nephritis group and the purpura nephritis group was significantly higher than that of the healthy control group (P0.05). There was no significant difference in the H2R expression level between the non nephritis group and the glomerulonephritis group (P0.05). The allergic purpura non nephritis group and the nephritis group were H4R (H4R). The level of expression was significantly higher than that of healthy controls (P0.05). There was no significant difference in H4R expression between the two groups of non nephritis and nephritis (P0.05).
2, the expression of histamine H2R before and after treatment with histamine H2 Receptor Antagonist Cimetidine in children with acute Henoch Schonlein purpura.
The mRNA level of H2R before treatment in cimetidine group was (1.77 + 0.51). The mRNA level of H2R before treatment in non cimetidine group was (1.82 + 0.50), mRNA level of H2R in healthy control group was (1.22 + 0.18). The level of H2R in cimetidine group and non cimetidine group before treatment was higher than that of healthy control group (P0.01). But cimetidine group and non cimetidine group treated H2R m before treatment. There was no significant difference in RNA expression (P0.05). The mRNA level of H2R in cimetidine group was (1.07 + 0.05) after treatment. The mRNA level of H2R after treatment in non cimetidine group was (1.44 + 0.34). The mRNA expression level of H2R after treatment in two groups was significantly different (P0.05), and the H2RmRNA level of cimetidine treatment group changed greatly.
3, the level of serum IL-8 in Henoch Schonlein purpura
In the serum of children with anaphylactoid purpura, the content of IL-8 was (332.04 + 83.48) pg/ml, and the content of IL-8 in the serum of the children with the recovery period was (97.75 + 18.16) pg/ml. The serum IL-8 content in the healthy control group was (90.9 + 9.73) pg/ml. allergic purpura. The serum IL-8 level was significantly higher than that of the children with the anaphylactoid purpura. In children (P0.05), there was no significant difference in the level of children with Henoch Schonlein purpura at the recovery stage (P0.05).
The serum IL-8 content of the children with anaphylactoid purpura non nephritis group was (300.86 + 98.62) pg/ml, the content of IL-8 in the serum of the nephritis group was (359.56 + 57.24) pg/ml, and the level of IL-8 in the healthy control group was (90.9 + 9.73) pg/ml. nephritis group. The serum IL-8 level of the children in the non nephritis group was significantly higher than that of the healthy control group. The non kidney of the allergic purpura was not the kidney. There was significant difference in IL-8 content between the two groups of inflammation group and nephritis group (P0.05), and IL-8 level in nephritis group was higher than that in non nephritis group.
conclusion
1, the expression of histamine H2R, H4R protein and nRNA in the mononuclear cells of children with anaphylactoid purpura was significantly higher than that of the children with anaphylactoid purpura, and healthy children. The expression of H4R could be reduced to the level of healthy children after the treatment of symptomatic anaphylactoid purpura, and the expression of H4R could be reduced to the level of healthy children. Histamine H2R, H4R may be involved in the pathogenesis of anaphylactoid purpura.
2, in the acute phase of anaphylactoid purpura non nephritis group, the expression of histamine H2R, H4R protein and mRNA in the mononuclear cells of the nephritis group were all higher than that of the healthy children. There was no significant difference in the expression of H4R between the two groups of the anaphylactoid purpura non nephritis group and the nephritis group. There was no significant difference in the expression of the renal and histamine H4R in the Henoch Schonlein purpura.
3, in children with anaphylactoid purpura at acute stage, the changes of histamine H2R in the group of H2R receptor antagonist cimetidine treatment group were significantly different from those in the non cimetidine group before and after treatment. The expression of histamine receptor was reduced by cimetil Ding Ke.
4, the serum levels of IL-8 in children with anaphylactoid purpura were significantly higher than those in the recovery period of anaphylactoid purpura, and in healthy children, the content of IL-8 in the children with anaphylactoid purpura during the treatment of symptomatic remission could be reduced to the level of.IL-8 in healthy children and may be involved in the pathogenesis of anaphylactoid purpura.
5, the serum IL-8 level of children with Henoch Schonlein purpura nephritis is higher than that of non nephritis group, and IL-8 may be involved in the occurrence and development of purpuric nephritis.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R725.5

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