PCR和血清学检测在儿童EB病毒感染诊断中的意义

发布时间：2018-06-24 19:44

本文选题：EB病毒 + 血清学　；参考：《中华医院感染学杂志》2017年01期

【摘要】：目的探讨PCR与血清学检测在儿童EB病毒(EBV)感染性疾病诊断中的意义。方法选取2015年1月-2016年1月疑似EB病毒感染的住院患儿245例,根据年龄分为婴儿组、幼儿组、学龄前组和学龄组,采用化学发光免疫分析法进行血清VCA IgM、EA IgG及NA IgG的检测,同时采用实时荧光定量PCR法检测全血和血浆中EBV-DNA载量,并根据检测结果将患儿分为原发感染组、再激活组和既往感染组,比较不同年龄和不同感染组间EBV血清学和PCR法检测阳性率的差异。结果血浆EBV-DNA阳性率和拷贝数均明显低于全血(P=0.00);在原发感染组和再激活组,全血DNA阳性率均高于血浆DNA和血清学阳性率(P=0.00);按年龄组分类,血清学阳性率在婴儿组低于其他年龄组(χ2=12.38,P=0.00);在婴儿组、幼儿组和学龄前组,全血DNA阳性率均高于血清学(P0.05),在学龄组,两者差异无统计学意义。结论 PCR与血清学检测EBV感染的阳性率和意义不同,应尽量采用两种方法结合来检测儿童EBV感染。
[Abstract]:Objective to investigate the significance of PCR and serological detection in the diagnosis of Epstein-Barr virus (EBV) infection in children. Methods 245 hospitalized children with suspected Epstein-Barr virus infection from January 2015 to January 2016 were divided into infant group, preschool group and school age group according to their age. The serum VCA IgMEA IgG and na IgG were detected by chemiluminescence immunoassay. The EBV-DNA load in whole blood and plasma was detected by real-time fluorescence quantitative PCR, and the children were divided into three groups: primary infection group, reactivated group and previous infection group. To compare the positive rate of EBV serology and PCR between different age groups and different infection groups. Results the positive rate and copy number of EBV-DNA in plasma were significantly lower than those in whole blood (P0. 00), in primary infection group and reactivated group, the positive rate of whole blood DNA was higher than that in plasma DNA and serological positive rate (P0. 00). The positive rate of serum DNA in infant group was lower than that in other age group (蠂 2 + 12.38% P0. 00), in infant group, infant group and preschool group, the positive rate of whole blood DNA was higher than that in serology (P0.05), but there was no significant difference between them in school age group. Conclusion the positive rate and significance of PCR and serological detection of EBV infection are different. Two methods should be used to detect EBV infection in children.
【作者单位】：青岛市妇女儿童医院青岛大学附属青岛妇女儿童医院检验科;青岛市市北区镇江路街道社区卫生服务中心;
【分类号】：R725.1

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