DGCR8在先天性心脏病患者血液及心肌组织中的表达

发布时间：2018-07-14 19:24

【摘要】：目的:探讨迪乔治综合征危象区基因8(DGCR8)与先天性心脏病(CHD)发生的关系及临床意义。方法:收集CHD患儿及健康儿童血液样本各40份,采用qRT-PCR方法检测DGCR8 mRNA的表达;收集室间隔缺损(VSD)患儿心肌组织25份,法洛四联症(TOF)患儿心肌组织16份,采用qRT-PCR和Western blot方法检测DGCR8mRNA和蛋白的表达,分析间隔缺损患儿血液中DGCR8的表达水平与心脏间隔缺损大小的相关性。结果:与健康儿童相比,CHD患儿血液中DGCR8 mRNA的表达量降低(P=0.037);与VSD患儿相比,TOF患儿心肌组织中DGCR8 mRNA和蛋白的表达量降低(P0.05);DGCR8与心脏间隔缺损无明显相关性(rS=-0.022,P=0.917)。结论:DGCR8基因的缺失与CHD的发生有关,影响心脏的正常发育。
[Abstract]:Objective: to investigate the relationship between DGCR8 gene and congenital heart disease (CHD) and its clinical significance. Methods: the expression of DGCR8 mRNA was detected by qRT-PCR in 40 children with CHD and 40 healthy children, 25 patients with ventricular septal defect (VSD) and 16 children with tetralogy of Fallot (TOF). The expression of DGCR8 mRNA and protein was detected by qRT-PCR and Western blot. The correlation between the expression level of DGCR8 and the size of cardiac septal defect in children with septal defect was analyzed. Results: compared with healthy children, the expression of DGCR8 mRNA in the blood of CHD children was decreased (P0. 037), and the expression of DGCR8 mRNA and protein in myocardial tissue of children with TOF was lower than that in children with VSD (P0.05). There was no significant correlation between DGCR8 mRNA expression and cardiac septal defect (rS- 0.022P0.917). Conclusion the deletion of the DGCR8 gene is related to the occurrence of CHD and affects the normal development of the heart.
【作者单位】：郑州大学第三附属医院妇产科;田纳西大学健康科学中心病理医学实验室;河南省人民医院儿童心脏中心;河南省妇科肿瘤纳米医学国际联合实验室;
【基金】：国家自然科学基金面上项目81572574 河南省高校科技创新团队项目16IRTSTHN018 郑州大学创新项目DYCX053
【分类号】：R725.4

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