川崎病内皮祖细胞数量和血栓前体蛋白水平的变化及丹参对其影响
发布时间：2018-07-21 21:09
【摘要】：目的 测定川崎病(Kawasaki disease,KD)患儿体内内皮祖细胞(endothelial progenitor cells,EPCs)数量及血栓前体蛋白(thrombus precursor protein,TpP)水平的变化,并研究加用丹参注射液治疗后对川崎病患儿体内EPCs数量及TpP水平变化是否有影响。探讨川崎病内皮功能、血流动力学变化及丹参对川崎病的治疗作用。 方法 选取住院治疗的20例KD患儿,其中男11例,女9例,为川崎病组,再随机分成2组进行治疗,其中1组仅给予传统的丙种球蛋白2g/kg静滴冲击治疗1次,阿司匹林30-50mg/kg/d,热退3-5天后减量,潘生丁3-5mg/kg/d治疗,选为非丹参组；另一组在此基础上加用丹参注射液0.2-0.3ml/kg/次,1次／日,连用5天为丹参组。另选取门诊体检的10例健康小儿,其中男5例,女5例为对照组。川崎病组与对照组用药前空腹用抗凝管采肘静脉血3m1,分离出单核细胞和血浆,其中单核细胞经处理后采用流式细胞仪检测外周血EPCs的数量,血浆用双抗体夹心固相酶联免疫吸附法(ELI SA)检测TpP水平,并对其结果进行统计学分析,比较两组结果是否有统计学意义；丹参组和非丹参组与用药5天后空腹采血测外周血EPCs数量和TpP水平,比较用丹参和不用丹参治疗EPCs和TpP的变化是否具有统计学意义。 结果 ①川崎病组外周血中EPCs数量显著低于对照组(P0.05)； ②川崎病组血浆中TpP水平显著高于对照组(P0.05)； ③川崎病组经治疗后丹参组EPCs数量显著高于非丹参组(P0.05)； ④川崎病组经治疗后丹参组TpP水平显著低于非丹参组(P0.05)。 结论 川崎病组患儿测得外周血中EPCs数量较对照组明显减少,说明川崎病患儿体内生成EPCs减少或其消耗、破坏增多,提示存在内皮功能损伤且内皮修复及再生的能力下降；外周血TpP水平明显升高,提示与川崎病患儿血液处于高凝状态有关,具有形成血栓或心肌梗塞的危险；经治疗后EPCs数量及TpP水平较前均明显好转,提示川崎病经治疗后内皮功能和血液高凝状态均得到改善。丹参组EPCs数量较非丹参组明显增高,而TpP水平较非丹参组明显下降,提示加用丹参的治疗方法对EPCs数量和TpP水平的改善较不加丹参更明显,说明丹参具有增加川崎病患儿外周循环血EPCs数量及降低TpP水平的功能,从而发挥保护血管内皮、促进内皮修复和再生和抗凝等作用。祖国医学将其归结为活血化瘀功效。
[Abstract]:Objective to determine the number of endothelial progenitor cells (endothelial progenitor cells) and the level of thromboplastin (thrombus precursor protein (TP) in children with Kawasaki disease (KD). The effects of salvia miltiorrhiza injection on the number of EPCs and the level of TP in children with Kawasaki disease were studied. To investigate the changes of endothelial function and hemodynamics of Kawasaki disease and the therapeutic effect of Salvia miltiorrhiza on Kawasaki disease. Methods Twenty children with KD were randomly divided into two groups, including 11 males and 9 females, who were treated with Kawasaki disease. Among them, group 1 was treated with conventional intravenous infusion of gamma globulin (2g/kg) only once. Aspirin 30-50 mg / kg / d, reduced after 3 to 5 days of heat withdrawal, dipyridamole 3-5mg/kg/d was selected as non-salvia miltiorrhiza group, another group was treated with salvia miltiorrhiza injection 0.2-0.3ml/kg/ once a day for 5 days. In addition, 10 healthy children, including 5 males and 5 females, were selected as control group. In Kawasaki disease group and control group, 3ml blood was collected from elbow vein blood with anticoagulant tube on an empty stomach before treatment. Monocytes and plasma were isolated. Flow cytometry was used to detect the number of EPCs in peripheral blood. The plasma levels of TP were detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Eli SA), and the results were analyzed statistically. The number of EPCs and the level of TP in peripheral blood of Danshen group and non-salvia miltiorrhiza group were measured on an empty stomach after 5 days. The changes of EPCs and TpP were statistically significant between salvia miltiorrhiza group and non-salvia miltiorrhiza group. Results 1the number of EPCs in the peripheral blood of Kawasaki disease group was significantly lower than that in the control group (P0.05), and the plasma level of TP in Kawasaki disease group was significantly higher than that in the control group (P0.05). 3 the number of EPCs in the group of Kawasaki disease was significantly higher than that in the group without salvia miltiorrhiza (P0.05), and the level of TpP in the group of Kawasaki disease was significantly lower than that in the group without salvia miltiorrhiza (P0.05). Conclusion the number of EPCs in the peripheral blood of children with Kawasaki disease was significantly lower than that in the control group, which indicated that the EPCs production in children with Kawasaki disease was decreased or consumed, and the destruction was increased in children with Kawasaki disease. The results indicated that there were endothelial function damage and the ability of endothelial repair and regeneration decreased, and the level of TPP in peripheral blood was significantly increased, suggesting that the blood of children with Kawasaki disease was in a hypercoagulable state and was at risk of thrombosis or myocardial infarction. After treatment, the number of EPCs and the level of TP were significantly improved, suggesting that the endothelial function and blood hypercoagulability of Kawasaki disease were improved after treatment. The number of EPCs in the salvia miltiorrhiza group was significantly higher than that in the non-salvia miltiorrhiza group, while the level of TpP in the salvia miltiorrhiza group was significantly lower than that in the non-salvia miltiorrhiza group. These results suggest that salvia miltiorrhiza has the function of increasing the number of EPCs in peripheral circulation and decreasing the level of TP in the peripheral circulation of children with Kawasaki disease, so as to protect vascular endothelium, promote endothelial repair, regeneration and anticoagulation. Traditional Chinese medicine ascribes it to the effect of promoting blood circulation and removing blood stasis.
【学位授予单位】：湖北中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.4
本文编号：2136863
[Abstract]:Objective to determine the number of endothelial progenitor cells (endothelial progenitor cells) and the level of thromboplastin (thrombus precursor protein (TP) in children with Kawasaki disease (KD). The effects of salvia miltiorrhiza injection on the number of EPCs and the level of TP in children with Kawasaki disease were studied. To investigate the changes of endothelial function and hemodynamics of Kawasaki disease and the therapeutic effect of Salvia miltiorrhiza on Kawasaki disease. Methods Twenty children with KD were randomly divided into two groups, including 11 males and 9 females, who were treated with Kawasaki disease. Among them, group 1 was treated with conventional intravenous infusion of gamma globulin (2g/kg) only once. Aspirin 30-50 mg / kg / d, reduced after 3 to 5 days of heat withdrawal, dipyridamole 3-5mg/kg/d was selected as non-salvia miltiorrhiza group, another group was treated with salvia miltiorrhiza injection 0.2-0.3ml/kg/ once a day for 5 days. In addition, 10 healthy children, including 5 males and 5 females, were selected as control group. In Kawasaki disease group and control group, 3ml blood was collected from elbow vein blood with anticoagulant tube on an empty stomach before treatment. Monocytes and plasma were isolated. Flow cytometry was used to detect the number of EPCs in peripheral blood. The plasma levels of TP were detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Eli SA), and the results were analyzed statistically. The number of EPCs and the level of TP in peripheral blood of Danshen group and non-salvia miltiorrhiza group were measured on an empty stomach after 5 days. The changes of EPCs and TpP were statistically significant between salvia miltiorrhiza group and non-salvia miltiorrhiza group. Results 1the number of EPCs in the peripheral blood of Kawasaki disease group was significantly lower than that in the control group (P0.05), and the plasma level of TP in Kawasaki disease group was significantly higher than that in the control group (P0.05). 3 the number of EPCs in the group of Kawasaki disease was significantly higher than that in the group without salvia miltiorrhiza (P0.05), and the level of TpP in the group of Kawasaki disease was significantly lower than that in the group without salvia miltiorrhiza (P0.05). Conclusion the number of EPCs in the peripheral blood of children with Kawasaki disease was significantly lower than that in the control group, which indicated that the EPCs production in children with Kawasaki disease was decreased or consumed, and the destruction was increased in children with Kawasaki disease. The results indicated that there were endothelial function damage and the ability of endothelial repair and regeneration decreased, and the level of TPP in peripheral blood was significantly increased, suggesting that the blood of children with Kawasaki disease was in a hypercoagulable state and was at risk of thrombosis or myocardial infarction. After treatment, the number of EPCs and the level of TP were significantly improved, suggesting that the endothelial function and blood hypercoagulability of Kawasaki disease were improved after treatment. The number of EPCs in the salvia miltiorrhiza group was significantly higher than that in the non-salvia miltiorrhiza group, while the level of TpP in the salvia miltiorrhiza group was significantly lower than that in the non-salvia miltiorrhiza group. These results suggest that salvia miltiorrhiza has the function of increasing the number of EPCs in peripheral circulation and decreasing the level of TP in the peripheral circulation of children with Kawasaki disease, so as to protect vascular endothelium, promote endothelial repair, regeneration and anticoagulation. Traditional Chinese medicine ascribes it to the effect of promoting blood circulation and removing blood stasis.
【学位授予单位】：湖北中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.4
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