CD31、CD34和CD133蛋白在婴幼儿血管瘤组织中的表达及意义

发布时间：2018-08-03 18:24

【摘要】：目的观察CD31、CD34和CD133蛋白在婴幼儿血管瘤(IH)组织中的表达,并探讨其临床意义。方法收集IH患儿存档蜡块50例(增生期25例,消退期25例)、瘤旁正常皮肤组织5例,采用免疫组化SP法检测组织中内皮祖细胞(EPCs)标志物CD31、CD34和CD133蛋白,采用HPIAS-1000图文报告管理系统对CD31、CD34和CD133蛋白的表达进行定量分析。结果增生期血管瘤组织CD31、CD34和CD133蛋白的阳性面积率分别是23%±3%、19%±4%、37%±2%,积分光密度(IOD)分别是0.26±0.06、0.23±0.03、0.52±0.02,均高于消退期血管瘤组织和瘤旁正常皮肤组织(P均0.05),而消退期血管瘤组织和瘤旁正常皮肤组织中CD31、CD34和CD133蛋白的IOD和阳性面积率比较差异无统计学意义(P均0.05)。结论 EPCs标志物CD31、CD34和CD133蛋白在增生期血管瘤组织中高表达,其在血管瘤的发生发展中起着重要作用。
[Abstract]:Objective to investigate the expression of CD31, CD34 and CD133 protein in (IH) of infantile hemangioma and its clinical significance. Methods 50 cases of archived wax masses (25 cases in proliferative phase and 25 cases in extinction phase) and 5 cases of normal skin tissue adjacent to tumor were collected. Immunohistochemical SP method was used to detect the expression of CD31, CD34 and CD133 protein in endothelial progenitor cells (EPCs). The expression of CD31, CD34 and CD133 protein was quantitatively analyzed by HPIAS-1000 graphic and text report system. Results the positive area rates of CD31, CD34 and CD133 protein in proliferative hemangioma were 23% 卤3 and 19% 卤4, 37% 卤2, respectively. The integral optical density (IOD) was 0. 26 卤0. 06 卤0. 023 卤0. 03 卤0. 52 卤0. 02, respectively, which was higher than that in involutive hemangioma and normal skin tissue adjacent to tumor (P0. 05), but in involuted hemangioma and adjacent normal skin tissue (P0. 05), the integral optical density (IOD) was 0. 26 卤0. 06 卤0. 03 卤0. 02, respectively. There was no significant difference in IOD and positive area between CD31, CD34 and CD133 protein in normal skin tissue (P0. 05). Conclusion EPCs markers CD31, CD34 and CD133 are highly expressed in proliferative hemangioma and play an important role in the development of hemangioma.
【作者单位】：广州医科大学附属第五医院;广州医科大学;
【基金】：广东省企业技术研发与升级改造专项基金项目计划(2013B021800191)
【分类号】：R732.2

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