儿童急性淋巴细胞白血病SOCS3 mRNA的表达水平与临床特点、早期治疗反应的相关性

发布时间：2018-10-30 20:56

【摘要】：目的:观察细胞因子信号转导抑制因子-3(SOCS3 mRNA)在儿童急性淋巴细胞白血病(ALL)中的表达水平,分析SOCS3 mRNA表达水平高低与儿童ALL疾病状态、危险度之间的关系,探讨SOCS3 mRNA在儿童ALL疾病状态和危险度评估、靶向治疗中的应用。方法:采用SYBR Green荧光定量PCR的方法检测45例新诊断ALL患儿初诊时、诱导缓解期和13例正常儿童骨髓单个核细胞中SOCS3 mRNA表达水平;分别采用方差分析和秩和检验的方法分析SOCS3 mRNA表达水平与ALL危险度、临床特点的关系。同时采用免疫荧光组织化学染色联合激光共聚焦显微技术测定ALL患儿骨髓单核细胞中SOCS3 mRNA的位置与表达量。结果:45例患儿初诊时SOCS3mRNA表达水平明显低于正常儿童(P0.05);诱导缓解期45例患儿的SOCS3 mRNA表达水平与正常儿童无显著差异(P0.05);初诊时中、高危组患儿SOCS3 mRNA表达水平高于低危组(P0.05);按照中位数法将45例患儿初诊时SOCS3 mRNA表达水平分为高表达组和低表达组,比较2组患儿临床特点发现,SOCS3 mRNA高表达组具有更高的外周血白细胞计数、乳酸脱氢酶水平和预后不良基因。此外,2组在危险度比较时SOCS3 mRNA高表达组危险度更高。结论:SOCS3 mRNA在初诊时下调而在疾病诱导缓解后恢复正常表达,SOCS3可以作为评价疾病状态的指标。SOCS3 mRNA的高表达使ALL危险度上调,SOCS3 mRNA可以作为评价ALL危险度的因子,通过调节SOCS3 mRNA表达水平治疗ALL或许可以成为治疗的新方向。
[Abstract]:Objective: to observe the expression of cytokine signal transduction inhibitor 3 (SOCS3 mRNA) in (ALL) of childhood acute lymphoblastic leukemia (AML), and to analyze the relationship between the expression of SOCS3 mRNA and the disease status and risk of ALL in children. To explore the application of SOCS3 mRNA in the assessment of disease status and risk of ALL in children. Methods: the expression of SOCS3 mRNA in bone marrow mononuclear cells of 45 newly diagnosed ALL children and 13 normal children was detected by SYBR Green fluorescence quantitative PCR. ANOVA and rank sum test were used to analyze the relationship between SOCS3 mRNA expression level and ALL risk and clinical characteristics. Immunofluorescence histochemical staining and laser confocal microscopy were used to detect the location and expression of SOCS3 mRNA in bone marrow monocytes of children with ALL. Results: the expression of SOCS3mRNA in 45 children was significantly lower than that in normal children (P0.05), but there was no significant difference in SOCS3 mRNA expression between 45 children and normal children (P0.05). In the first visit, the expression of SOCS3 mRNA in high risk group was higher than that in low risk group (P0.05). According to the median method, 45 children were divided into high expression group and low expression group according to the median method. Comparing the clinical characteristics of the two groups, it was found that the high expression group of SOCS3 mRNA had higher peripheral blood leukocyte count. Lactate dehydrogenase level and poor prognosis gene. In addition, the risk of high expression of SOCS3 mRNA was higher in the two groups than in the risk group. Conclusion: SOCS3 mRNA is down-regulated at first visit and returns to normal expression after disease induction and remission. SOCS3 can be used as an index to evaluate the disease state. The high expression of SOCS3 mRNA can increase the risk of ALL, and SOCS3 mRNA can be used as a risk factor to evaluate the risk of ALL. The treatment of ALL by regulating the level of SOCS3 mRNA expression may be a new direction of treatment.
【作者单位】：安徽医科大学第二附属医院儿科;
【分类号】：R733.71

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