婴幼儿重症肺炎血清中MMP-9、TIMP-1的表达及意义
发布时间：2018-11-19 22:08
【摘要】：肺炎是儿科的常见疾病,由于婴幼儿本身的解剖和生理特点,呼吸系统发育不成熟,极容易进展为重症肺炎,目前,重症肺炎仍是我国婴幼儿死亡的重要原因。细胞因子基质金属蛋白酶9(Matrix metalloproteinases-9,MMP-9)及基质金属蛋白酶抑制剂1(Tissue Inhibitors of Matrix Metalloproteinase-1,TIMP-1)是参与细胞外基质新陈代谢的主要酶类。近年来关于MMP-9、TIMP-1与呼吸系统疾病的关系日益受到重视。研究发现,MMP-9及其抑制剂TIMP-1在呼吸系统炎症性疾病中发挥了一定作用,肺部炎症性疾病时,MMP-9、TIMP-1的分泌量及活性有改变,本研究通过观察婴幼儿肺炎时,外周血血清中MMP-9、TIMP-1水平的变化,以及恢复期潮气呼吸肺功能的情况,探讨细胞因子MMP-9、TIMP-1与肺炎严重程度、不同病原学以及恢复期潮气呼吸肺功能情况的相关性,推断细胞因子MMP-9、TIMP-1与疾病严重程度及预后的关系,以便为临床早期诊断、治疗及治疗后评估提供客观依据。研究目的探讨婴幼儿重症肺炎急性期外周血血清中MMP-9、TIMP-1的表达及临床意义。研究方法选取2015年10月至2016年5月于郑州大学第三附属医院重症监护病房住院的婴幼儿重症肺炎20例为重症肺炎组,同期在本院普通呼吸病区住院的婴幼儿轻症肺炎25例为轻症肺炎组;另依据病原学将上述所选取的45例患儿分为病毒组24例和非病毒组21例;20例同期健康体检儿童为对照组。患儿入住病区后立即抽取外周静脉血3-4ml,高速离心取上清液置于-80℃冰箱内保存待检测,采用酶联免疫吸附法(ELISA)法测定外周血中MMP-9和TIMP-1水平。另待患儿病情临床治愈出院前,行潮气呼吸肺功能检查。结果1急性期肺炎患儿外周血血清中细胞因子MMP-9、TIMP-1和MMP-9/TIMP-1水平均显著高于对照组,重症肺炎组高于轻症肺炎组,病毒组高于非病毒组(均P0.05);2恢复期肺炎组患儿肺功能达峰时间比(TPTEF/TE)和达峰容积比(VPTEF/VE)均明显低于对照组,重症肺炎组明显低于轻症肺炎组,病毒组低于非病毒组(均P0.05);但患儿每公斤体重潮气量(VT/Kg)的各组间比较无显著差异。3外周血血清中MMP-9水平及MMP-9/TIMP-1比值与肺功能参数TPTEF/TE、VPTEF/VE均呈负相关(r1=-0.459,-0.376;r2=-0.413,-0.327;均P0.05)。结论婴幼儿肺炎急性期外周血血清中MMP-9及MMP-9/TIMP-1水平可反映疾病严重程度、病原体种类和肺功能损伤的程度,可作为判断预后的客观指标。
[Abstract]:Pneumonia is a common disease in pediatrics. Because of the anatomical and physiological characteristics of infants and young children, the respiratory system is immature, and it is easy to develop into severe pneumonia. At present, severe pneumonia is still an important cause of infant mortality in China. Cytokine matrix metalloproteinase 9 (Matrix metalloproteinases-9,MMP-9) and matrix metalloproteinase inhibitor 1 (Tissue Inhibitors of Matrix Metalloproteinase-1,TIMP-1 are the main enzymes involved in extracellular matrix metabolism. In recent years, more and more attention has been paid to the relationship between MMP-9,TIMP-1 and respiratory diseases. It was found that MMP-9 and its inhibitor TIMP-1 played a certain role in inflammatory diseases of the respiratory system. The secretion and activity of MMP-9,TIMP-1 were changed in patients with pulmonary inflammatory diseases. The changes of serum MMP-9,TIMP-1 level in peripheral blood and the respiratory and pulmonary function of tidal air during convalescence were studied to explore the relationship between cytokine MMP-9,TIMP-1 and the severity of pneumonia. The relationship between cytokine MMP-9,TIMP-1 and the severity and prognosis of the disease was inferred in order to provide objective basis for early clinical diagnosis, treatment and post-treatment evaluation. Objective to investigate the expression and clinical significance of MMP-9,TIMP-1 in peripheral blood of infants with severe pneumonia. Methods from October 2015 to May 2016, 20 infants with severe pneumonia in the intensive care unit of the third affiliated Hospital of Zhengzhou University were selected as the severe pneumonia group. At the same time, 25 cases of mild pneumonia in the common respiratory area of our hospital were treated with mild pneumonia. According to etiology, 45 children were divided into viral group (n = 24) and non-viral group (n = 21) and healthy children (n = 20) as control group. The peripheral venous blood was extracted from the patients at 3 ~ 4 ml. The supernatant was stored in the refrigerator at -80 鈩，
本文编号：2343567
[Abstract]:Pneumonia is a common disease in pediatrics. Because of the anatomical and physiological characteristics of infants and young children, the respiratory system is immature, and it is easy to develop into severe pneumonia. At present, severe pneumonia is still an important cause of infant mortality in China. Cytokine matrix metalloproteinase 9 (Matrix metalloproteinases-9,MMP-9) and matrix metalloproteinase inhibitor 1 (Tissue Inhibitors of Matrix Metalloproteinase-1,TIMP-1 are the main enzymes involved in extracellular matrix metabolism. In recent years, more and more attention has been paid to the relationship between MMP-9,TIMP-1 and respiratory diseases. It was found that MMP-9 and its inhibitor TIMP-1 played a certain role in inflammatory diseases of the respiratory system. The secretion and activity of MMP-9,TIMP-1 were changed in patients with pulmonary inflammatory diseases. The changes of serum MMP-9,TIMP-1 level in peripheral blood and the respiratory and pulmonary function of tidal air during convalescence were studied to explore the relationship between cytokine MMP-9,TIMP-1 and the severity of pneumonia. The relationship between cytokine MMP-9,TIMP-1 and the severity and prognosis of the disease was inferred in order to provide objective basis for early clinical diagnosis, treatment and post-treatment evaluation. Objective to investigate the expression and clinical significance of MMP-9,TIMP-1 in peripheral blood of infants with severe pneumonia. Methods from October 2015 to May 2016, 20 infants with severe pneumonia in the intensive care unit of the third affiliated Hospital of Zhengzhou University were selected as the severe pneumonia group. At the same time, 25 cases of mild pneumonia in the common respiratory area of our hospital were treated with mild pneumonia. According to etiology, 45 children were divided into viral group (n = 24) and non-viral group (n = 21) and healthy children (n = 20) as control group. The peripheral venous blood was extracted from the patients at 3 ~ 4 ml. The supernatant was stored in the refrigerator at -80 鈩，
本文编号：2343567
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