川崎病患儿血浆中人软骨糖蛋白39的变化及临床意义

发布时间：2018-11-21 15:00

【摘要】：目的:通过研究川崎病(Kawasaki disease,KD)患儿各期血浆中人软骨糖蛋白39(Human Cartilage glycoprotein 39,HC gp-39)的表达水平及其与冠状动脉损伤的关系,探讨HC gp-39对KD诊断及病情评估的临床意义。方法:于2015年3月至2016年2月选取在山西省儿童医院心脏与风湿免疫科确诊为KD的患儿50例作为KD组,每例根据疾病的演变过程分为急性期和亚急性期,同时将KD组的急性期根据彩色多普勒超声心动图结果分为冠状动脉损伤组(CAL)17例和无冠状动脉损伤组(NCAL)33例,将同时段来其门诊体检的健康儿童20例作为健康对照组。采用双抗体夹心固相酶联免疫吸附法检测所有究对象血浆中HC gp-39的表达水平,通过各组间的比较及相关性分析来探究川崎病患儿血浆中HC gp-39的表达水平及其与冠状动脉损伤的关系。结果:定量检测表明KD组各期血浆HC gp-39的表达水平均高于健康对照(F=94.42P0.05);KD组的急性期血浆HC gp-39的表达水平高于亚急性期(P0.05);KD组在急性期CAL组血浆HC gp-39的表达水平明显高于NCAL组(t=4.39 P0.05);相关性分析显示,患儿血浆中HC gp-39与血沉(r=0.78,P0.05)、血小板(r=0.80,P0.05)、C反应蛋白(r=0.71,P0.05),均呈正相关。结论:人软骨糖蛋白39在川崎病的发生发展中可能起到一定的作用,动态检测血浆中HC gp-39可能对KD的早期诊断及病情评估有重要的临床意义。
[Abstract]:Objective: to investigate the expression of human chondroglycoprotein 39 (Human Cartilage glycoprotein 39 HC gp-39 in plasma of children with Kawasaki disease (Kawasaki disease,KD) and its relationship with coronary artery injury. To explore the clinical significance of HC gp-39 in the diagnosis and evaluation of KD. Methods: from March 2015 to February 2016, 50 children with KD diagnosed in the Department of Cardiac and Rheumatological Immunology, Shanxi Children's Hospital, were selected as KD group. Each patient was divided into acute stage and subacute stage according to the evolution of the disease. At the same time, according to the results of color Doppler echocardiography, KD group was divided into coronary artery injury group (n = 17) and coronary artery injury group (n = 33). 20 healthy children who were examined at the same time were used as healthy control group. The expression of HC gp-39 in plasma was detected by double antibody sandwich solid phase enzyme-linked immunosorbent assay (Elisa). To explore the expression level of HC gp-39 and its relationship with coronary artery injury in children with Kawasaki disease (Kawasaki disease). Results: quantitative analysis showed that the level of plasma HC gp-39 expression in KD group was higher than that in healthy control group (F=94.42P0.05); KD group was higher than that in subacute phase (P0.05). The expression of plasma HC gp-39 in KD group was significantly higher than that in NCAL group in acute phase CAL group (tr 4.39 P0.05). Correlation analysis showed that there was a positive correlation between plasma HC gp-39 and erythrocyte sedimentation rate (r = 0.78P), platelet (r = 0.80), C reactive protein (P 0.05). Conclusion: human cartilage glycoprotein 39 may play a role in the occurrence and development of Kawasaki disease. Dynamic detection of HC gp-39 in plasma may play an important role in the early diagnosis and evaluation of KD.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.4

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