2012～2014年中国西藏自治区5岁以下健康儿童肠道病毒携带状况分析

发布时间：2019-01-17 20:22

【摘要】：了解西藏自治区2012~2014年5岁以下健康儿童粪便标本中携带肠道病毒的血清型分布及流行特点。采集西藏自治区三地(拉萨、日喀则和山南地区)5岁以下健康儿童的粪便标本,同时收集相关流行病学信息。使用RD和L20B细胞进行病毒分离,将阳性分离株用肠道病毒分子定型法进行血清型鉴定。2012~2014年从西藏自治区5岁以下健康儿童中共采集了460份粪便标本(每名儿童采集1份),分离到肠道病毒共133株,包括脊髓灰质炎(脊灰)疫苗株33株,肠道病毒分离率为28.91%,非脊灰肠道病毒的分离率为21.74%。西藏自治区健康人群肠道病毒携带率和携带病毒的型别存在年代和地域的差异,但均以EV-B为主。其中在2012~2014年中,每年非脊灰肠道病毒谱中CV-B3是分离数量最多的。2012~2014年西藏5岁以下健康儿童中没有检出脊灰野病毒和脊灰疫苗衍生病毒,维持了无脊灰状态。检出了多种非脊灰肠道病毒,CV-B3一直在西藏自治区尤其是拉萨市健康儿童中隐性传播。本研究不仅为我国维持无脊灰工作提供坚实的实验室依据,同时,对非脊灰肠道病毒的流行本底的研究,为西藏自治区非脊灰肠道病毒的暴发提供预测预警信息和科技支撑。
[Abstract]:To investigate the serotype distribution and epidemic characteristics of enterovirus in fecal samples of healthy children under 5 years old from 2012 to 2014 in Tibet Autonomous region. Feces of healthy children under 5 years of age were collected from three areas of Tibet Autonomous region (Lhasa, Xigaze and Shannan), and epidemiological information was collected. RD and L20B cells were used to isolate the virus, and the positive strains were identified by enterovirus molecular typing. A total of 460 fecal specimens (1 for each child) were collected from healthy children under 5 years of age in Tibet Autonomous region from 2012 to 2014. A total of 133 strains of enterovirus were isolated, including 33 strains of poliomyelitis vaccine, the isolation rate of enterovirus was 28.91 and the isolation rate of non-polio enterovirus was 21.74. The prevalence and type of enterovirus in healthy population in Tibet Autonomous region were different in age and region, but EV-B was the main factor. From 2012 to 2014, CV-B3 was the most isolated virus in the spectrum of non-polio enterovirus. No wild poliovirus and polio-vaccine-derived virus were detected in healthy children under 5 years of age in Tibet from 2012 to 2014, maintaining polio-free status. Many non-polio enterovirus have been detected and CV-B3 has been transmitted recessive in Tibetan Autonomous region especially among healthy children in Lhasa. This study not only provides a solid laboratory basis for maintaining polio free work in China, but also studies the epidemic background of non-polio enterovirus, and provides early warning information and scientific and technological support for the outbreak of non-polio enterovirus in Tibet Autonomous region.
【作者单位】：西藏自治区疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所脊灰实验室国家卫生计生委医学病毒与病毒病重点实验室;
【基金】：国家自然科学基金(项目号:81672070),题目:引起大规模流行的CV-A6的进化及其导致重症手足口病的分子机制研究
【分类号】：R725.1

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