不同剂量重组人促红细胞生成素诱导小鼠继发性细胞增多症模型研究
[Abstract]:In order to study the effect of different doses of recombinant human erythropoietin (rhEpo) on the establishment of secondary erythrocythemia mouse model in Balb/c female mice, and to provide a high-efficient and high-quality service platform for the development of new drugs for this disease, Balb/c female mice aged 8-10 weeks were randomly divided into Saline- negative control group, rhEpo-3IU-4d group, rhEpo-10IU-4d group, rhEpo-10IU-7d group, rhEpo-10IU-14d group and rhEpo-30IU-4d group on the 3rd day before the experiment. In the rhEpo-10IU-4d recovery group, 24 hours after the last subcutaneous injection of rhEpo, the mice were euthanized and the blood and tissues were collected. The reticulocyte count, hematocrit and spleen weight were measured. The results showed that the number of reticulocytes, hematocrit and spleen weight increased significantly with the increase of rhEpo dosage and injection times. In rhEPO-10IU-4d group, reticulocyte number increased 5.9 times and hematocrit increased by more than 10%. The hematocrit increased further in rhEpo-10IU-14d group, but the reticulocyte number and spleen weight did not continue to increase. It is suggested that continuous subcutaneous injection of different doses of rhEpo can induce different degrees of secondary hypercythemia.
【作者单位】: 上海交通大学农业与生物学院/上海市兽医生物技术重点实验室;科文斯医药研发(上海)有限公司;
【分类号】:R-332;R722.1
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