急性毛细支气管炎患儿血清IL-17水平变化与临床意义探讨

发布时间：2019-03-18 12:26

【摘要】：目的研究急性期毛细支气管炎的免疫病理状态。方法选择46例急性期毛细支气管炎患儿(其中轻症组24例,重症组22例)、20例普通病毒性肺炎患儿和15例健康婴儿为研究对象,酶联免疫吸附法测定血清IL-17、IFN-γ水平,化学发光法测定血清IgE水平。结果(1)各组间患儿血清IL-17水平的比较：急性期毛细支气管炎患儿血清IL-17水平为(26.91±7.13)ng/L,较普通病毒性肺炎组[(21.96±5.62)ng/L]和对照组[(13.56±5.18)ng/L]均显著升高,差异有显著意义(q=4.04、9.81,P0.01)；普通病毒性肺炎组患儿血清IL-17水平较对照组也显著升高,差异有显著意义(q=5.37,P0.01)；急性期毛细支气管炎轻症组患儿血清IL-17水平[(26.54±7.93)ng/L]与重症组[(27.32±6.31)ng/L]比较差异无显著意义(t=0.37,P=0.71)。 (2)各组间患儿血清IFN-γ水平的比较：急性期毛细支气管炎患儿血清IFN-γ水平为(7.07±1.86)ng/L,较普通病毒性肺炎组[(9.12±1.89)ng/L]和对照组[(8.95±1.64)ng/L]显著降低,差异有极显著意义(q=5.91、4.89,P0.01)；而普通病毒性肺炎组血清IFN-γ水平较对照组差异无显著意义(q=0.38,P0.05)。急性期毛细支气管炎重症组患儿血清IFN-γ水平[(6.13±1.56)ng/L]较轻症组[(7.93±1.72)ng/L]明显降低,差异有显著意义(t=3.7,P0.01)。 (3)各组间患儿血清IgE水平的比较：急性期毛细支气管炎患儿血清IgE水平[(171.77±58.19)IU/ml]较普通病毒性肺炎组[(51.38±12.44)IU/ml]及对照组[(43.95±12.81)IU/ml]均显著升高,差异有显著意义(q=18.08、18.65,P0.01)；急性期毛细支气管炎重症组患儿血清1gE水平[(189.27±48.49)IU/m]]较轻症组[(155.73±62.56)IU/ml]明显减低,差异有显著意义(t=2.02,P0.05)。结论毛细支气管炎患儿急性期Thl细胞活性受抑、Th17细胞功能显著增强,在其发病机制中发挥重要作用。
[Abstract]:Objective to study the immunopathologic status of acute bronchiolitis. Methods 46 children with acute bronchiolitis (24 cases in mild group and 22 cases in severe group), 20 children with common viral pneumonia and 15 healthy infants were selected as subjects. Serum IL-17, was measured by enzyme linked immunosorbent assay (Elisa). The level of IFN- 纬 was measured by chemiluminescence method. The serum IgE level was determined by chemiluminescence. Results (1) the serum IL-17 levels in children with acute bronchiolitis were (26.91 卤7.13) ng/L, and (26.91 卤7.13) ng/L, respectively. Compared with the common viral pneumonia group [(21.96 卤5.62) ng/L] and the control group [(13.56 卤5.18) ng/L], the difference was significant (Q = 4.04, 9.81, P0.01). The level of serum IL-17 in the common viral pneumonia group was significantly higher than that in the control group (Q = 5.37, P0.01). There was no significant difference in serum IL-17 level between acute bronchitis group [(26.54 卤7.93) ng/L] and severe group [(27.32 卤6.31) ng/L] (t = 0.37, P = 0.71). (2) comparison of serum IFN- 纬 levels in children with acute bronchiolitis: the serum IFN- 纬 levels in children with acute bronchiolitis were (7.07 卤1.86) ng/L,. Compared with the normal viral pneumonia group [(9.12 卤1.89) ng/L] and the control group [(8.95 卤1.64) ng/L], the difference was very significant (Q = 5.91, 4.89, P0.01). There was no significant difference in the level of serum IFN- 纬 between the common viral pneumonia group and the control group (Q = 0.38, P0.05). The serum IFN- 纬 level in acute bronchitis group [(6.13 卤1.56) ng/L] was significantly lower than that in mild group [(7.93 卤1.72) ng/L], the difference was significant (t = 3.7, P0.01). (3) comparison of serum IgE levels in children with acute bronchiolitis [(171.77 卤58.19) IU/ml] compared with common viral pneumonia group [(51.38 卤12.44) IU/ml] and the control group [(51.38 卤12.44) IU/ml]. [(43.95 卤12.81) IU/ml] were significantly increased. The difference was significant (Q = 18.08, 18.65, P0.01); The serum 1gE level in acute bronchiolitis group [(189.27 卤48.49) IU/m] was significantly lower than that in mild group [(155.73 卤62.56) IU/ml], the difference was significant (t = 2.02, P0.05). Conclusion in the acute stage of bronchiolitis, the activity of Thl cells is inhibited and the function of Th17 cells is significantly enhanced, which plays an important role in the pathogenesis of bronchiolitis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.6

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