过敏性紫癜患儿T细胞受体保守域α链基因多态性研究
发布时间：2019-04-21 21:09
【摘要】：目的 探讨T细胞受体保守域α链基因(TCRCα)-560C/T,575A/G位点多态性与过敏性紫癜(HSP)发病之间的关系。 方法 应用聚合酶链反应-限制内切酶片段长度多态分析(PCR-PFLP)方法结合琼脂糖凝胶电泳技术,对36例过敏性紫癜患儿和60例正常对照组儿童进行TCRCα-560C/T,575A/G位点基因多态性分析。 结果 (1) TCRCα基因-560位点的CC、CT、TT三种基因型频率在紫癜组和正常组中比较无显著性差异(x2=5.39,P0.05),而该位点的等位基因频率C和T在紫癜组和正常组之间差异有显著意义(x2=4.69,P0.05)。 (2)TCRCα基因-575位点的AA、AG、GG三种基因型频率在紫癜组和正常组中比较无显著性差异(x2=1.07,P0.05),该位点的等位基因频率A和G在紫癜组和正常组差异无显著性(x2=1.01,P0.05)。 结论 (1) TCRCα-560C/T基因多态性变化与HSP的发生可能存在一定关联。 (2)TCRCα-575A/G基因多态性变化与HSP的发生无明显关联性。
[Abstract]:Aim to investigate the relationship between T cell receptor conserved domain 伪 chain gene (TCRC 伪) 560C / T, 575A 伪 G polymorphism and the pathogenesis of (HSP) in Henoch-Schonlein purpura (Henoch-Schonlein purpura). Methods 36 children with Henoch-Schonlein purpura (Henoch-Schonlein purpura) and 60 normal children were studied with polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-PFLP) combined with agarose gel electrophoresis. Polymorphism analysis of 575A/G locus gene. Results (1) there was no significant difference in the frequencies of three genotypes of CC,CT,TT at site-560of TCRC 伪 gene between purpura group and normal group (x2, 5.39, P0.05). The allele frequencies C and T of this locus were significantly different between purpura group and normal group (x2 / 4.69, P0.05). (2) there was no significant difference in the frequencies of AA,AG,GG genotypes at site-575 of TCRC 伪 gene between purpura group and normal group (x2? 1.07, P0.05). There was no significant difference in allele frequencies A and G between Henoch-Schonlein purpura group and normal group (x2? 1.01, P0.05). Conclusion (1) the polymorphism of TCRC 伪-560C/T gene may be associated with the occurrence of HSP. (2) there was no significant correlation between the polymorphism of TCRC 伪-575A/G gene and the occurrence of HSP.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.5
本文编号：2462558
[Abstract]:Aim to investigate the relationship between T cell receptor conserved domain 伪 chain gene (TCRC 伪) 560C / T, 575A 伪 G polymorphism and the pathogenesis of (HSP) in Henoch-Schonlein purpura (Henoch-Schonlein purpura). Methods 36 children with Henoch-Schonlein purpura (Henoch-Schonlein purpura) and 60 normal children were studied with polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-PFLP) combined with agarose gel electrophoresis. Polymorphism analysis of 575A/G locus gene. Results (1) there was no significant difference in the frequencies of three genotypes of CC,CT,TT at site-560of TCRC 伪 gene between purpura group and normal group (x2, 5.39, P0.05). The allele frequencies C and T of this locus were significantly different between purpura group and normal group (x2 / 4.69, P0.05). (2) there was no significant difference in the frequencies of AA,AG,GG genotypes at site-575 of TCRC 伪 gene between purpura group and normal group (x2? 1.07, P0.05). There was no significant difference in allele frequencies A and G between Henoch-Schonlein purpura group and normal group (x2? 1.01, P0.05). Conclusion (1) the polymorphism of TCRC 伪-560C/T gene may be associated with the occurrence of HSP. (2) there was no significant correlation between the polymorphism of TCRC 伪-575A/G gene and the occurrence of HSP.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.5
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