促红细胞生成素与黄芪对新生儿窒息后血清诱导人肾小管细胞TLR 4 /MyD88表达的影响

发布时间：2021-07-25 12:41

　　目的：研究促红细胞生成素与黄芪对新生儿窒息后血清诱导人肾小管细胞TLR4/MyD88 （Toll-like receptors 4/myeloid differentiation factor 88）表达的影响方法：（1）以HK-2细胞（Human renal proximal tubular cell,人近曲肾小管上皮细胞）作为研究对象。（2）以足月重度窒息（生后Apgar评分均小于4分）新生儿第一个24小时血清（20%体积分数）作为致病因素。（3）实验分四组,即对照组、窒息组、EPO（erythropoietin,促红细胞生成素）组、黄芪（Astragalus, AS）组。（4）检测指标：倒置相差显微镜下观察HK-2细胞形态变化；流式细胞仪（Flow Cytometry, FCM）检测各组细胞TLR4特异性荧光抗体阳性结合率表达情况；采用免疫组织化学法（SP法）检测各组细胞MyD88表达情况。（5）统计学分析：数据均用x±s表示,采用one-way ANOVA分析,组间比较用LSD法,运用SPSS14.0软件进行统计学处理,P<0.05为差异具有统计学意义。结果：（1）倒置相... 

【文章来源】：西南医科大学四川省

【文章页数】：65 页

【学位级别】：硕士

【部分图文】：

EPO和黄蔑对新生儿窒息后血清诱导人CDHK--2细胞形态学变化的影响(光

附图图1EPO和黄蔑对新生儿窒息后血清诱导人镜X400)。CDHK--2细胞形态学变化的影响(光A:对照组B:窒息组C:EPO组D:黄蔑组Figure1EffeetofErythroPoietinandAstr昭alusontheehangesofmorPhology(IH，underC:groupinH恤anHK--2indueedbyPostasPhyxial一ser恤ofneonateslightmieroseoPyX400).A:eontrolgrouP，B:asPhyxiagrouP，Astragalus.PretreatlllentwithEPO，D:grouPofPretreatment贾ith

Astragalus.一一。八八八.八八.../反反反反反反反反反一一一一洲火火，‘’耐认’志图2EPO和黄蔑对新生儿窒息后血清诱导人HKesZ细胞TL凡特异性荧光抗体阳性结合率表达的影响A:对照组B:窒息组c:EPO组D:黄蔑组Figure2EffeetsofErythroPoietinandAstragalusontheexPressionofthePositivebindingrateofsPeeialfluoreseentantibodyinTLR;onHKesZeellindueedbypostasphyxial一serumofneonates(F以).A:eontrolgroup，B:asPhyxiagrouP，C:grouPofPretreat.entwithEPOD:grouPofPretreat.ent下ithAstragalus.

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