动态增强MR定量监测血管靶向药物治疗结肠癌移植瘤

发布时间：2018-01-07 04:41

本文关键词：动态增强MR定量监测血管靶向药物治疗结肠癌移植瘤　出处：《中国医学影像技术》2016年06期 　论文类型：期刊论文

【摘要】：目的应用动态增强MR(DCE-MRI)参数定量监测血管靶向类药物治疗结肠癌移植瘤的早期效果。方法将36只接种人结肠癌HT-29细胞的裸鼠按随机数字法等分为3组,并分别腹腔注射贝伐单抗(抗血管生成药物组,10mg/kg体质量)、考布他汀(血管破坏剂组,100mg/kg体质量)和0.9%氯化钠溶液(对照组,100mg/kg体质量)。分别于给药前和给药后1、24、48h对实验动物行DCE-MRI扫描,分析传输常数(Ktrans)、回流常量(Kep)、血管外细胞外容积分数(Ve)和增强曲线下初始面积(iAUC)。每次扫描完成后各组随机处死2只裸鼠,行HE染色及免疫组织化学染色检测瘤体微血管密度(MVD)。DCE-MRI参数组间及组内不同时间的比较行方差分析;DCE-MRI参数与MVD间的关系行Pearson相关性分析。结果裸鼠皮下结肠癌移植瘤瘤体边缘区域DCE-MRI呈显著的渐进性强化,呈高灌注,中心区域强化幅度较低,呈现低灌注改变;抗血管生成药物组和血管破坏剂组不同时间点Ktrans及Kep值差异均有统计学意义(P均0.05);对照组肿瘤的Ktrans值差异无统计学意义(P=0.60),Kep值差异均有统计学意义(P=0.01);Ktrans和Kep值治疗前组间比较差异均无统计学意义(P均0.05)。治疗后1、24、48h,相同时间Ktrans和Kep值3组间比较差异均有统计学意义(P均0.05);不同时间点抗血管生成药物组Ve值差异有统计学意义(P=0.03),治疗后24h组间比较Ve和iAUC值差异均有统计学意义(P均0.05);抗血管生成药物组和血管破坏剂组坏死较对照组明显,血管内皮较对照组稀疏。MVD计数与Ktrans和Kep值呈正相关(r=0.66、0.71,P均0.05)。结论 DCE-MRI参数Ktrans和Kep能及时准确地监测到肿瘤血管靶向药物治疗后的效果,或可指导临床方案的制定。
[Abstract]:Objective to apply dynamic enhanced Mr DCE-MRI (DCE-MRI). Methods 36 nude mice inoculated with human colon cancer HT-29 cells were divided into 3 groups according to random number method. Bevacizumab (10 mg / kg body mass) and cobuvastatin (vascular disruptor group) were injected intraperitoneally respectively. 100 mg / kg body mass) and 0.9% mg / kg sodium chloride solution (control group 100 mg / kg body weight). The experimental animals were scanned with DCE-MRI for 48 h, and the transport constants were analyzed. The extracellular volume fraction (Veg) and the initial area under the enhancement curve were measured. After each scanning, 2 nude mice were randomly killed in each group. The microvessel density of the tumor was detected by HE staining and immunohistochemical staining. The parameters of MVDX. DCE-MRI were compared between and within the group by ANOVA. The relationship between DCE-MRI parameters and MVD was analyzed by Pearson. Results DCE-MRI in the marginal region of subcutaneous colon cancer transplanted tumor in nude mice showed significant progressive enhancement. There were high perfusion and low enhancement in the central region. The values of Ktrans and Kep in antiangiogenic drug group and vascular disrupting agent group were significantly different at different time points (P < 0.05). There was no significant difference in Ktrans value between the control group and the control group. There was no significant difference in Ktrans and Kep between the two groups before treatment (P < 0.05). There were significant differences in Ktrans and Kep between the three groups at the same time (P < 0.05). There were significant differences in ve values between the two groups at different time points (P < 0.05), and there were significant differences in ve and iAUC values between the two groups 24 hours after treatment (P < 0.05). The necrosis of the anti-angiogenic drugs group and the vascular disrupting agent group was more obvious than that of the control group. The number of vascular endothelial thinning. MVD was positively correlated with the values of Ktrans and Kep. Conclusion DCE-MRI parameters Ktrans and Kep can be used to monitor the effect of vascular targeted drug therapy in time, or to guide the formulation of clinical protocols. [WT5HZ] [WT5 "HZ] [WT5" BZ] [WT5 "BZ]
【作者单位】：成都大学附属医院放射科;新疆医科大学第二附属医院影像中心;新疆医科大学研究生学院;
【分类号】：R735.35;R445.2
【正文快照】： 熊青青1,段玉梅1,杨朝炳1,王云玲2,贾文霄3*(1.成都大学附属医院放射科,四川成都610081;2.新疆医科大学第二附属医院影像中心,新疆乌鲁木齐830011;3.新疆医科大学研究生学院,新疆乌鲁木齐830011)肿瘤血管的分子靶向治疗以瘤体血管和(或)其形成系统为靶点,有选择地作用在既存血

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