微血管密度在前列腺癌和慢性前列腺炎中表达与超声血流动力学的对比研究

发布时间：2018-02-09 04:50

  本文关键词： 前列腺癌 慢性前列腺炎 超声血流动力学 微血管密度　出处：《广西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：本研究旨在通过经直肠彩色多普勒超声对前列腺可疑病灶区血流动力学变化的观察,并结合与病理微血管密度(microvascular denstity,MVD)的免疫组化观察,探讨经直肠彩色多普勒超声鉴别诊断前列腺癌和慢性前列腺炎的诊断价值。 方法：收集临床疑为前列腺癌患者共61例,经直肠超声扫查前列腺,检测前列腺可疑病灶区血流动力学指数(Vs、Vd、PI、RI),并进行血运分级(0～3级)。并于超声引导下行前列腺穿刺,获取病理结果证实为前列腺癌38例,慢性前列腺炎23例。点阵列包埋、石蜡切片行HE染色,经病理医师诊断,用CD34单克隆抗体对前列腺活检组织行免疫组织化学染色,采用Weinder方法对CD34标记的微血管密度进行定量计数。 结果：前列腺癌组病灶区血流动力学指数(Vs、PI、RI)及血运分级明显高于慢性前列腺炎组(P0.05)；Vs、PI、RI各指标均在前列腺癌(C+D)期和(A+B)期、前列腺癌Gleason评分分为≥7分高Gleason评分组和7分低Gleason评分组两组中比较有差异性(P均0.05)。血流动力学指数Vd于各组比较中均无明显差异(P0.05)。MVD计数在前列腺癌组和慢性前列腺炎组分别为46.70±13.87、34.38±7.28(P0.05)。MVD计数在前列腺癌(C+D)期和(A+B)期分别为56.99±12.85、39.97±10.21(P0.05)；在前列腺癌高Gleason评分组和低Gleason评分组分别为53.79±13.30、36.96±7.24(P0.05)。前列腺癌病灶区的血流动力学指数(Vs、PI、RI)及血运分级与前列腺癌组织MVD呈显著正相关(P0.05)。 结论：经直肠彩色多普勒超声影像显示的前列腺癌血流动力学指数(Vs、PI、RI)和血运分级能较好地反映肿瘤组织内微血管的变化,且前列腺癌组可疑病灶或血流丰富区血流动力学指数(Vs、PI、RI)及血运分级明显高于慢性前列腺炎组,可作为两者鉴别诊断的有用指标。
[Abstract]:Objective: to observe the changes of hemodynamics in the suspected lesion area of prostate by transrectal color Doppler ultrasound and to observe the microvascular denstitystitystitystitystitystitystitystityus microvascular VDD by immunohistochemistry. To evaluate the diagnostic value of transrectal color Doppler ultrasound in the differential diagnosis of prostate cancer and chronic prostatitis. Methods: a total of 61 patients with suspected prostate cancer were collected. The prostate was examined by transrectal ultrasound, and the hemodynamic index of the suspected lesions was detected. Pathological results were obtained from 38 cases of prostate cancer and 23 cases of chronic prostatitis. The specimens were embedded with dot array and stained with HE on paraffin sections. The biopsy tissues were stained with CD34 monoclonal antibody by immunohistochemical staining after being diagnosed by pathologist. The microvessel density (MVD) labeled with CD34 was counted quantitatively by Weinder method. Results: the regional hemodynamic index (Pi) and the grade of blood circulation in prostate cancer group were significantly higher than those in chronic prostatitis group (P 0.05). The Gleason score of prostate cancer was divided into two groups: high score (鈮，

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