STZ诱导大鼠1型糖尿病进行性脑萎缩的磁共振成像及组织化学研究

发布时间：2018-02-27 06:12

本文关键词： 磁共振成像(MRI) 型糖尿病(TDM) 链脲佐菌素组织化学染色进行性脑萎缩　出处：《波谱学杂志》2015年03期 　论文类型：期刊论文

【摘要】：1型糖尿病(T1DM)是一种慢性代谢疾病,主要表现为胰岛素分泌量较正常情况下降,会对人体的多个器官和系统造成持续性的损伤.关于糖尿病的横向研究发现糖尿病患者相比于正常人存在着显著的脑萎缩,但关于糖尿病引起的脑萎缩随时间发生进行性改变的研究比较少见.实验采用腹腔注射链脲佐菌素(STZ)来诱导建立大鼠的1型糖尿病模型,运用磁共振成像(MRI)的方法对萎缩的脑区进行定位并在造模后12周和20周两个时间点对脑萎缩的程度进行对比分析,然后运用组织化学染色的方法观察在MRI上出现进行性萎缩的脑区中的神经元所发生的病理改变.MRI的结果表明:STZ诱导的T1DM大鼠相比于正常对照组大鼠出现了显著性的全脑体积、灰质体积和白质体积的萎缩,并且在多个白质脑区和灰质脑区均出现了萎缩程度随着病程的延长而逐渐加重.组织化学染色的结果发现,STZ诱导的T1DM大鼠相对于正常对照组大鼠在体感皮层、运动皮层和海马CA3区,均出现明显的神经元萎缩现象.
[Abstract]:Type 1 diabetes mellitus (T1DM) is a chronic metabolic disease characterized by a decrease in insulin secretion. Sustained damage to multiple organs and systems of the human body. Horizontal studies of diabetes have found that people with diabetes have significant brain atrophy compared with normal people. However, studies on the progressive changes of brain atrophy caused by diabetes over time are rare. The rat model of type 1 diabetes was induced by intraperitoneal injection of streptozotocin (STZ). Magnetic resonance imaging (MRI) was used to locate the atrophy brain regions and to compare the degree of brain atrophy at 12 and 20 weeks after modeling. Then the pathological changes of neurons in the brain area with progressive atrophy on MRI were observed by histochemical staining. The results of MRI showed that the brain volume of T1DM rats induced by 1: STZ was significantly higher than that of normal control rats. The atrophy of gray matter and white matter, The degree of atrophy in several white matter and gray matter brain regions was gradually aggravated with the prolongation of the course of disease. Histochemical staining showed that STZ-induced T1DM rats were in somatosensory cortex as compared with normal control rats. There were obvious neuronal atrophy in CA3 area of motor cortex and hippocampus.
【作者单位】：中国科学院生物磁共振分析重点实验室波谱与原子分子物理国家重点实验室武汉磁共振中心(中国科学院武汉物理与数学研究所);中国科学院大学;
【分类号】：R587.1;R445.2

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