rhKGF、CBLB502和WR2721对放射性口腔黏膜炎的防治效果观察

发布时间：2018-03-19 16:16

本文选题：辐射损伤　切入点：实验性　出处：《解放军医学杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体重变化,另外20只小鼠利用1%甲苯胺蓝染色法观察照射小鼠舌组织溃疡情况,HE染色观察舌组织病理学改变,Ki-67免疫组化检测黏膜角化上皮细胞增殖情况。结果与照射对照组相比,rh KGF和WR2721给药组小鼠30d存活率显著提高,体重恢复较快,角化上皮细胞增殖显著,未发生明显的口腔黏膜炎;CBLB502组小鼠的存活率、体重变化及组织病理学指标与照射对照组比较差异无统计学意义(P0.05)。结论 rh KGF和WR2721对放射性口腔黏膜炎有较好的防治作用,而CBLB502并不能减轻放射性口腔黏膜炎的发生。
[Abstract]:Objective to compare the preventive and therapeutic effects of r h KG FG CB CB B 502W R 2721 on radioactive oral mucositis. Methods healthy male C57BL / 6J mice were randomly divided into normal control group. The 30 day survival rate and body weight of 17Gy head and neck irradiated mice were observed in the control group, rh KGF group, CBLB502 group and WR2721 group. The other 20 mice were stained with 1% toluidine blue to observe the ulcer of tongue tissue in irradiated mice. The pathological changes of tongue tissue were observed by HE staining and Ki-67 immunohistochemistry was used to detect the proliferation of keratinized epithelial cells of mucous membrane. Results compared with the control group, the proliferation of keratinocytes was detected. Compared with Rh KGF and WR2721 groups, the 30-day survival rate of mice was significantly increased. The recovery of body weight was faster, the proliferation of keratinocytes was significant, and the survival rate of CBLB502 mice without oral mucositis was significant. There was no significant difference in body weight and histopathology between the two groups. Conclusion Rh KGF and WR2721 have better preventive and therapeutic effects on radiation oral mucositis, but CBLB502 can not reduce the occurrence of radiation oral mucositis.
【作者单位】：新乡医学院研究生院;军事医学科学院放射与辐射医学研究所;武警总医院消化内科;
【分类号】：R730.55

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