靶向超声造影剂和普通超声造影剂对卵巢癌新生血管的评价

发布时间：2018-03-20 12:37

本文选题：靶向超声造影剂　切入点：卵巢癌　出处：《新疆医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：本研究通过制备携血管内皮生长因子受体2单克隆抗体(VEGFR2)的靶向超声造影剂(MBt),通过对比普通超声造影剂(MBc)与MBt对裸鼠卵巢癌SKOV3移植瘤新生血管的超声造影显像,探讨两种造影剂对裸鼠卵巢癌移植瘤新生血管的应用价值。方法：裸鼠卵巢癌SKOV3细胞株皮下移植瘤模型的建立；采用“生物素-亲和素桥连接”法制备靶向超声造影剂；采用免疫荧光法观察超声微泡表面VEGFR2单克隆抗体的结合情况；荷瘤鼠分别注射MBt与MBc行超声造影检查并分析靶向组与普通组的时间强度曲线(TIC)相关参数；免疫组织化学检测肿瘤组织新生血管的VEGFR2的表达情况。结果：成功建立裸鼠卵巢癌SKOV3细胞株皮下移植瘤模型；免疫荧光法在荧光显微镜下观察到微泡超声表面发出的明亮的绿色荧光;靶向组与普通组时间强度曲线(TIC)分析结果如下：靶向组的峰值强度73.95(69.50,83.08)dB较普通组57.55(54.30.74.10)dB高(P0.01),靶向组的持续时间480.00(442.50,617.50)S较普通组354.00(297.50,412.50)S更长(P0.01)；免疫组织化学结果显示肿瘤组织内新生血管的VEGFR2表达。结论：携VEGFR2单克隆抗体的靶向超声造影剂能够与裸鼠卵巢癌移植瘤新生血管内皮特异性结合并实现靶向分子的超声显像,故靶向超声造影技术对卵巢癌新生血管形成的评估提供了新方法。
[Abstract]:Objective: in this study, we prepared the target ultrasound contrast agent of VEGFR2 carrying vascular endothelial growth factor receptor 2 (VEGFR2), and compared the conventional ultrasound contrast agent (MBC) with that of MBt in detecting the neovascularization of SKOV3 transplanted tumor of ovarian cancer in nude mice. To evaluate the value of two contrast agents in the treatment of ovarian cancer xenografts in nude mice. Methods: the model of xenograft tumor in nude mice ovarian cancer SKOV3 cell line was established, and the targeted ultrasound contrast agent was prepared by "biotin-affinity bridge connection" method. Immunofluorescence method was used to observe the binding of monoclonal antibody to VEGFR2 on the surface of ultrasound microbubbles, the tumor bearing mice were injected with MBt and MBc, respectively, and the time intensity curves of target group and normal group were analyzed. The expression of VEGFR2 in tumor tissue was detected by immunohistochemistry. Results: the model of subcutaneous transplantation of ovarian cancer SKOV3 cell line in nude mice was successfully established. The bright green fluorescence from the ultrasonic surface of microbubbles was observed under the fluorescence microscope by immunofluorescence method. The time intensity curves of target group and normal group were as follows: the peak intensity of target group was 73.95 ~ 69.50 ~ 83.08 dB higher than that of normal group (57.55 ~ 54.30.74.10 dB), and the duration of target group was 480.00N 442.50 ~ 617.50S longer than that of normal group (354.00 ~ 297.50 ~ 412.50), and the result of immunohistochemistry showed that the neoplastic tissue was new in tumor tissue. Conclusion: targeting ultrasound contrast agent with VEGFR2 monoclonal antibody can specifically bind to neovascularization endothelium of ovarian cancer xenografts in nude mice and achieve targeted molecular ultrasound imaging. Therefore, targeted contrast-enhanced ultrasonography provides a new method for the evaluation of neoangiogenesis in ovarian cancer.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.1

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