经皮经肝超声造影显像评估兔VX2肝癌腹腔淋巴结转移的实验研究

发布时间：2018-03-25 00:37

本文选题：经皮超声造影　切入点：肝癌　出处：《山东大学学报(医学版)》2015年06期

【摘要】：目的探讨经皮经肝超声造影显像在评估兔VX2肝癌腹腔淋巴转移中的应用价值。方法新西兰大白兔30只,分为正常对照组(A组)5只,肝癌种植组(B组)25只,同时在癌组织及癌周组织注射超声造影剂Sono Vue,动态观察正常肝组织、癌组织及癌周组织的增强特点,并使用超声仪器自带造影分析软件定量分析。开腹清扫淋巴结并送病理学检查。结果 25只肝癌种植组(B组)中,无淋巴结转移(B1组)14只,淋巴结转移(B2组)11只。肝脏经皮经肝超声造影显像分为组织间隙期、弥散期、平台期、消退期4期,癌组织与癌周组织比较,超声造影剂在癌组织呈现为"快退",癌周组织能够持续增强20 min以上并逐渐消退。平台期强度(PTI)表现为癌组织、癌周组织及正常肝组织间差异有统计学意义(P0.01),且PTI癌组织PTI癌周组织PTI正常肝组织。B1、B2组癌周组织及A组正常肝组织各组间的PTI差异均有统计学意义(P0.01),且PTIB2PTIB1PTIA。B1组、B2组癌组织的PTI与A组比较差异有统计学意义(P0.05),但B1、B2两组间差异无统计学意义。结论经皮经肝超声造影显像可提供肝癌腹腔淋巴转移情况的信息,为选择治疗方式及分析预后提供重要依据。
[Abstract]:Objective to evaluate the value of percutaneous transhepatic ultrasound imaging in evaluating the abdominal lymphatic metastasis of VX2 hepatoma in rabbits. Methods Thirty New Zealand white rabbits were divided into normal control group (n = 5) and hepatoma implantation group (n = 25). At the same time, ultrasound contrast agent Sono Vue was injected into cancer tissue and pericancerous tissue to observe the enhancement characteristics of normal liver tissue, cancer tissue and pericancerous tissue dynamically. The lymph nodes were dissected by laparotomy and sent to pathological examination. Results in group B of 25 patients with liver cancer, 14 were in group B with no lymph node metastasis, 14 in group B without lymph node metastasis, and 14 in group B without lymph node metastasis. There were 11 cases in group B _ 2 of lymph node metastasis. The liver was divided into 4 stages by percutaneous transhepatic contrast-enhanced ultrasound imaging: interphase, diffuse stage, plateau stage, and extinction phase. The cancer tissue was compared with the pericancerous tissue. Ultrasound contrast agent showed "rapid regression" in cancer tissue, which could be continuously enhanced for more than 20 min and gradually receded. There was significant difference between the pericancerous tissues and normal liver tissues (P 0.01), and there was significant difference in PTI between the surrounding tissues of PTI and the normal liver tissues of group A and the surrounding tissues of PTI in PTI tissues of PTI cancer tissues, and the PTI of normal liver tissues in group A was significantly different from that in group PTIB2PTIB1PTIA.B1, and there was a significant difference in PTI in group B 2 and group B 2 in PTIB2PTIB1PTIA.B1 group. The difference of PTI between group A and group A was statistically significant (P 0.05), but there was no significant difference between group B 1 and B 2. Conclusion Percutaneous transhepatic contrast-enhanced imaging can provide information of lymphatic metastasis in abdominal cavity of hepatocellular carcinoma. To provide an important basis for the selection of treatment methods and analysis of prognosis.
【作者单位】：济宁市第一人民医院超声科;济宁市传染病医院超声科;泰安市中心医院特检科;济南军区总医院超声科;
【基金】：山东省自然科学基金面上项目(ZR2013HM026)
【分类号】：R445.1;R735.7

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