弥散加权成像定量评价非压迫性神经根炎微创治疗价值的实验研究

发布时间：2018-04-15 03:20

本文选题：腰椎间盘突出 + 弥散加权成像　；参考：《川北医学院》2014年硕士论文

【摘要】：目的：探讨弥散加权成像技术能否对腰椎非压迫性神经根炎不同微创治疗术后变化进行定量评价。材料与方法：中国小型猪12只CT导向下非压迫性神经根炎造模成功14天后，根据治疗方法不同随机分为4组，，每组3只实验动物，包括：A臭氧治疗组、B消炎液治疗组、C胶原酶治疗组、D对照组。分别于实验猪的L2~3、L3~4及L4~5双侧神经根旁硬膜外注入相应物质进行治疗。术前常规行MRI冠状位T2WI及DWI序列扫描。每组分别于术后第3天、7天及15天对实验猪进行冠状位T2WI及DWI扫描，扫描完成后处死1只实验动物，取相应双侧神经根行CD8阳性T淋巴细胞免疫组化染色。分析各组于术前、术后3天、7天、15天神经根ADC值变化及神经根CD8阳性T淋巴细胞计数评分的变化；以及神经根ADC值与神经根CD8阳性T淋巴细胞计数评分的相关性。结果：A、B、C、D组治疗前、治疗后3天、7天、15天神经根平均ADC值均出现下降趋势（P0.05）；A、B、C、D组治疗后3天、7天、15天神经根CD8阳性T淋巴细胞计数评分均出现下降趋势（P0.05）。A、B、C、D组神经根ADC值与神经根淋巴细胞计数定量评分均呈正相关，相关系数分别为rs为0.806、0.916、0.889、0.644，P值均0.05；所有观测淋巴细胞计数的神经根ADC值与神经根淋巴细胞计数定量评分呈正相关，相关系数rs为0.905，P0.05。结论：不同治疗组均能较好的使神经根炎得到改善，并且弥散加权成像技术能对腰椎非压迫性神经根炎微创治疗术后变化进行定量评价。
[Abstract]:Objective: to evaluate the effect of diffusion-weighted imaging (DWI) on the postoperative changes of lumbar noncompressed neuritis after different minimally invasive treatment.Materials and methods: twelve Chinese miniature pigs were randomly divided into 4 groups according to different treatment methods, 3 experimental animals in each group, after 14 days of successful modeling of non-compressed nerve root inflammation guided by CT.C collagenase treatment group and D control group.The corresponding substances were injected into the peridural space of the bilateral nerve roots of pig L2 and L4 5, respectively.MRI coronal T2WI and DWI sequences were performed before operation.In each group, coronal T2WI and DWI were performed on the 3rd day and 15th day after operation. After the scan, one animal was killed and the corresponding bilateral nerve roots were taken for CD8 positive T lymphocyte immunohistochemical staining.The changes of ADC value of nerve root and the score of CD8 positive T lymphocyte count in nerve root were analyzed before and 3 days after operation and 7 days after operation, and the correlation between ADC value of nerve root and CD8 positive T lymphocyte count score of nerve root was analyzed.Results before treatment, before treatment,The average ADC value of nerve root on the 3rd day, 7th day and 15th day after treatment all showed a downward trend. The ADC value of nerve root and the fine lymphoid of nerve root were decreased in group P0.05 and ANABU C + D on the 3rd day and 7th day after treatment and 15 days after treatment.The quantitative score of cell count was positively correlated.The correlation coefficients were r _ s 0.806 ~ 0.916 ~ 0.889U ~ (0.889) ~ 0.644m P = 0.05.The ADC value of nerve root was positively correlated with the quantitative score of nerve root lymphocyte count (r _ s = 0.905) (r _ s = 0.905) (P < 0.05), and the correlation coefficient (r _ s) was 0.905 (P ~ (0.05)).Conclusion: different treatment groups can improve nerve root inflammation, and diffusion weighted imaging can be used to quantitatively evaluate the changes of lumbar vertebrae non-compression neuritis after minimally invasive treatment.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.2;R681.5

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