低频超声增强万古霉素对导管表皮葡萄球菌生物膜影响的体内实验

发布时间：2018-04-15 04:17

本文选题：低频超声 + 万古霉素　；参考：《重庆医科大学》2014年硕士论文

【摘要】：【目的】建立兔导管相关性表皮葡萄球菌（S．epidermidis）生物膜（biofilm,BF）感染模型，探讨体内低频超声联合万古霉素对表皮葡萄球菌BF的干预作用。【方法】建立表皮葡萄球菌BF体外模型，将导管植入兔皮下，建立兔表皮葡萄球菌导管BF感染模型，分为空白对照组，单独低频超声组，单独万古霉素组，，低频超声联合万古霉素组。涂板菌落形成单位（CFU）计数法计算导管细菌数；导管表皮葡萄球菌BF结构的改变通过扫描电镜（SEM）来观察；皮肤、心脏、肝脏及肾脏病理组织切片检查炎症反应。【结果】成功建立导管表皮葡萄球菌感染的兔模型。各组导管log10(CFU/ml)计数结果分别：空白对照组(6.157±0.046)/ml，单独低频超声组(6.165±0.072)/ml，单独万古霉素组(5.195±0.047)/ml，低频超声联合万古霉素组(4.842±0.058)/ml。说明单独低频超声对BF内细菌没有杀灭作用，单独万古霉素能有效地杀灭BF内细菌，与空白对照组相比CFU减少，p＜0.01；低频超声联合万古霉素组效果更加明显。SEM结果显示低频超声对BF结构无影响；与各单独作用组相比，低频超声联合万古霉素能明显地破坏BF，使其结构变得更加疏松。皮肤病理组织切片显示低频超声联合万古霉素组，皮下隧道炎症明显减轻。空白对照组、单独低频超声组、单独万古霉素组，低频超声联合万古霉素组心脏、肝脏及肾脏组织未见异常。【结论】低频超声无法破坏表皮葡萄球菌BF结构，万古霉素对表皮葡萄球菌BF结构有一定的破坏。低频超声联合万古霉素能够更显著的清除导管表皮葡萄球菌BF结构，说明低频超声能够增强万古霉素对BF结构的破坏及细菌的杀灭能力。
[Abstract]:[objective] to establish a rabbit model of catheter-associated staphylococcus epidermidis biofilm (BFF) infection and to investigate the effect of low frequency ultrasound combined with vancomycin on staphylococcus epidermidis BF in vivo.[methods] the model of staphylococcus epidermidis BF in vitro was established, and the infective model of staphylococcus epidermidis was established by implanting the catheter under rabbit skin. The model was divided into blank control group, low frequency ultrasound group and vancomycin group.Low frequency ultrasound combined with vancomycin group.The bacterial count of catheterization was calculated by CFU method, the changes of BF structure of Staphylococcus epidermidis were observed by scanning electron microscope (SEM), and the inflammatory reaction was detected by pathological sections of skin, heart, liver and kidney.[results] the rabbit model of staphylococcus epidermidis infection was established successfully.The count results of CFU / ml in each group were as follows: the blank control group (6.157 卤0.046) / ml, the low frequency ultrasonic group (6.165 卤0.072) / ml, the vancomycin group (5.195 卤0.047) / ml, the low frequency ultrasound combined with vancomycin group (4.842 卤0.058) / ml respectively.The results showed that low frequency ultrasound alone did not kill bacteria in BF, and vancomycin alone could effectively kill bacteria in BF.Compared with the blank control group, CFU decreased P < 0.01, the effect of low frequency ultrasound combined with vancomycin was more obvious. The results showed that low frequency ultrasound had no effect on BF structure.Low frequency ultrasound combined with vancomycin can significantly destroy BFs and make their structures more loose.The pathological sections of skin showed that the subcutaneous tunnel inflammation was alleviated obviously in low frequency ultrasound combined with vancomycin group.There were no abnormalities in heart, liver and kidney in blank control group, single low frequency ultrasound group, single vancomycin group and low frequency ultrasound combined vancomycin group.[conclusion] low frequency ultrasound can not destroy the BF structure of Staphylococcus epidermidis, and vancomycin can destroy the BF structure of Staphylococcus epidermidis.Low frequency ultrasound combined with vancomycin could significantly remove staphylococcus epidermidis BF structure, indicating that low frequency ultrasound could enhance vancomycin's ability to destroy BF structure and kill bacteria.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R445.1;R965

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