连续和脉冲高强度聚焦超声辐照离体牛晶状体致凝固性坏死的变化过程

发布时间：2018-05-21 03:06

本文选题：高强度聚焦超声消融术 + 占空比　；参考：《中国介入影像与治疗学》2015年06期

【摘要】：目的观察连续高强度聚焦超声(CHIFU)与脉冲高强度聚焦超声(PHIFU)辐照离体牛晶状体所致凝固性坏死的变化过程,分析占空比对高强度聚焦超声(HIFU)辐照结果的影响。方法将80个离体牛晶状体分为4组行HIFU辐照,声功率均为500W,脉冲重复频率均为4Hz,A组(CHIFU组),占空比100%,辐照时间1s;B组,占空比50%,辐照时间2s;C组,占空比20%,辐照时间5s;D组,占空比10%,辐照时间10s。以高速摄影设备和被动空化采集(PCD)系统记录凝固性坏死的发生过程和焦域处发出的声散射信息,测算各组凝固性坏死体积,并对空化行为进行分析。结果 A组(CHIFU组)凝固性坏死出现的平均时间为0.41s,B、C、D组分别为0.93、2.22、6.28s;CHIFU辐照凝固性坏死出现时间早于PHIFU辐照。各组辐照后形成的凝固性坏死形态有所不同。A组(CHIFU组)凝固性坏死体积随辐照时间呈线性增长,B、C、D组凝固性坏死体积均呈非线性增长。辐照结束后,A组(CHIFU组)凝固性坏死体积为(12.99±2.11)mm3,B组为(12.69±1.79)mm3,C组为(12.09±1.93)mm3,D组为(6.94±1.54)mm3。D组凝固性坏死体积均小于其余3组(P均0.05),A、B、C组间两两比较差异无统计学意义(P均0.05)。各组均有空化发生,A组(CHIFU组)幅度均方根值(RMS)曲线有突然增大的过程,B、C、D组RMS曲线呈周期性的变化,占空比越高,空化行为越剧烈。结论CHIFU与PHIFU辐照所致牛晶状体凝固性坏死发生发展过程不同,调节HIFU辐照的占空比可影响凝固性坏死出现的时间及体积。
[Abstract]:Objective to observe the changes of coagulation necrosis induced by continuous high intensity focused ultrasound (CHIFU) and pulsed high intensity focused ultrasound (PHIFU) irradiation in vitro bovine lens and to analyze the effect of duty cycle on the results of high intensity focused ultrasound (HIFU) irradiation. Methods 80 isolated bovine lens were divided into 4 groups: HIFU irradiation with sound power of 500W, pulse repetition frequency of 4Hz A group and CHIFU group with duty cycle of 100, irradiation time of 1s ~ (-1) B, duty cycle of 50, irradiation time of 2 s ~ (-1) C, duty cycle of 20 times, irradiation time of 5 sD group, irradiation time of 2 sg C group, irradiation time of 5 sD group. The duty cycle is 10 s and the irradiation time is 10 s. High speed photography equipment and passive cavitation acquisition system were used to record the process of coagulation necrosis and the acoustic scattering information from the focal region. The volume of coagulation necrosis was measured and the cavitation behavior was analyzed. Results the mean time of coagulation necrosis in group A was 0.41 s-1 / c group (0.93 ~ 2.226.28 s-1) respectively. The time of coagulation necrosis in group A was earlier than that in group A (0.41 s-1) and that in group D was earlier than that in group C (0.93 ~ 2.222.226.28 s-1). The morphology of coagulation necrosis after irradiation was different. (group A) the volume of coagulation necrosis increased linearly with the irradiation time. After irradiation, the volume of coagulation necrosis in group A was 12.99 卤2.11mm ~ (3) and that in group B was 12.69 卤1.79 mm ~ (3) and that in group C was 12.09 卤1.93 卤1.93 mm ~ (3) respectively. The volume of coagulation necrosis in group A was lower than that in group C (P < 0.05). There was no significant difference between group B and group C (P = 0.05). The root-mean-square (RMS) curve of group A (group A) increased suddenly and the RMS curve of group B (C) D showed periodic changes. The higher the ratio of duty to duty, the more violent the cavitation behavior. Conclusion the process of coagulation necrosis of bovine lens induced by CHIFU and PHIFU irradiation is different. Adjusting the duty cycle of HIFU irradiation can influence the time and volume of coagulation necrosis.
【作者单位】：重庆医科大学生物医学工程学院省部共建超声医学工程国家重点实验室超声医学工程重庆市重点实验室;
【基金】：国家自然科学基金(11274404) 国家重点基础研究发展计划(973计划)项目(2011CB707900)
【分类号】：R445.1

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