经皮超声造影示踪兔乳腺引流淋巴结的实验研究

发布时间：2018-09-07 08:42

【摘要】：目的评价经皮超声造影对兔乳腺引流淋巴结的示踪效果,探讨超声造影指导乳腺癌前哨淋巴结活检术的可行性。方法 20只兔(40侧乳腺)根据超声造影剂剂量不同分为4组(0.25、0.50、0.75、1.00 ml),乳头周围皮下注射造影剂后观察淋巴显像,然后美蓝示踪,将检出淋巴结切除并行组织病理学检查。结果经皮超声造影可以清晰地动态观察兔淋巴引流并存在显影时间窗。电镜下可于显影淋巴结内观察到造影剂微气泡。超声造影平均每侧腋窝检出1.09枚淋巴结,美蓝平均每侧腋窝检出1.16枚淋巴结,超声造影与美蓝的淋巴结检出率没有统计学差异(87.5%vs 80%,P0.05)。结论实时超声造影可识别前哨淋巴结,经皮超声造影联合美蓝淋巴示踪可用于指导乳腺前哨淋巴结活检。
[Abstract]:Objective to evaluate the tracer effect of percutaneous contrast-enhanced sonography (PCEC) on mammary gland drainage lymph nodes in rabbits and to explore the feasibility of sentinel lymph node biopsy (SLN) guided by contrast-enhanced ultrasonography. Methods Twenty rabbits (40 sides of mammary glands) were divided into 4 groups according to the dose of ultrasound contrast medium (0.250.500.5050.05 ~ 1.00 ml),). Lymphatic imaging was observed after subcutaneous injection of contrast agent around the nipple, then methylene blue tracer was used to detect lymph node resection and histopathological examination. Results Percutaneous contrast-enhanced ultrasonography could clearly and dynamically observe lymphatic drainage in rabbits and there was a time window for development. Microbubbles of contrast media could be observed under electron microscope in developing lymph nodes. The average number of lymph nodes was 1.09 in each axilla and 1.16 in methylene blue. There was no significant difference between ultrasonography and methylene blue (87.5%vs 80 P 0.05). Conclusion Sentinel lymph nodes can be identified by real-time contrast-enhanced ultrasound. Percutaneous contrast-enhanced ultrasonography combined with methylene blue lymphatic tracer can be used to guide sentinel lymph node biopsy of mammary gland.
【作者单位】：北京大学人民医院;
【分类号】：R445.1;R737.9

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