长脉冲超声联合脂质体微泡溶解微血栓的体外实验研究

发布时间：2019-05-09 12:58

【摘要】：目的评价长脉冲超声联合脂质体微泡造影剂溶解微血栓的疗效。方法构建评价溶栓效能的体外循环系统,通过造成200~300μm的微血栓阻塞于滤网来模仿体内的微循环栓塞。然后治疗组给予脂质体微泡加超声治疗15min,超声频率1MHz、声压1.5MPa、脉冲间隔3s,包括100、1 000、5 000 3种周期,对照组给予超声照射但无声学微泡。结果治疗组溶栓的效果随着脉冲的延长而增加,且对应的惯性空化效应强度也随之增加。1 000和5 000周期组的溶栓率显著高于单纯超声组(P均0.01)。结论长脉冲超声联合脂质体微泡能实现良好的溶栓效果,且在一定范围内溶栓的效果随着脉冲的延长而增加。惯性空化可能是长脉冲超声介导微泡溶栓的重要机制之一。
[Abstract]:Objective to evaluate the efficacy of long pulse ultrasound combined with liposomes microbubble contrast agent in dissolving microthrombus. Methods an extracorporeal circulatory system was constructed to evaluate thrombolytic efficacy. The microthrombus of 200 ~ 300 渭 m was blocked in the filter mesh to imitate the microcirculation embolism in vivo. Then the treatment group was treated with liposome microbubbles plus ultrasound for 15 minutes, the ultrasound frequency was 1 MHz, the sound pressure was 1.5 MPA, the pulse interval was 3 s, including 100, 1 000, 5 000 cycles, and the control group was irradiated with ultrasound without acoustic microbubbles. Results the thrombolytic effect of the treatment group increased with the prolongation of the pulse and the intensity of the corresponding inertial cavitation effect also increased. The thrombolytic rate of the 1,000 and 5000 cycles group was significantly higher than that of the ultrasound group (P 0.01). Conclusion long pulse ultrasound combined with liposome microbubbles can achieve a good thrombolytic effect, and the thrombolytic effect increases with the prolongation of the pulse in a certain range. Inertial cavitation may be one of the important mechanisms of microbubble thrombolysis mediated by long pulse ultrasound.
【作者单位】：暨南大学附属第一医院心内科;韩国忠北健康科学大学;南方医科大学附属南方医院心内科;
【基金】：国家自然科学基金(No.81400266) 暨南大学科研培育与创新基金(No.21613310)
【分类号】：R445.1

【参考文献】