人胎盘间充质干细胞移植修复大鼠卵巢早衰的实验研究

发布时间：2017-12-26 17:36

本文关键词：人胎盘间充质干细胞移植修复大鼠卵巢早衰的实验研究　出处：《世界最新医学信息文摘》2016年90期 　论文类型：期刊论文

【摘要】：目的为探讨尾静脉注射移植人胎盘间充质干细胞(hp MSCs)对化疗导致的卵巢早衰模型大鼠卵巢功能的修复作用。方法经鉴定的人胎盘间充质干细胞经尾静脉注射移植给卵巢早衰模型大鼠,实验分为hp MSCs组和干细胞培养液移植组(control组),每组10只,分别通过尾静脉等体积注射,注射后观察大鼠卵巢结构损伤情况,比较血清性激素(E2、AMH、INHB)浓度。结果大鼠卵巢激素水平与control组相比,hpMSCs移植前后血清E2和INHB水平有统计学差异;血清AMH水平无统计学差异(P=0.051);大鼠卵巢组织形态学结果表明,与模型组大鼠卵巢皮质损伤严重,尾静脉注射hpMSCs后Bcl-2表达显著降低。结论 hAMSCs大鼠尾静脉移植改善激素水平,减少卵巢细胞凋亡,从而修复卵巢功能。
[Abstract]:Objective to investigate the repair effect of human placental mesenchymal stem cells (HP MSCs) on ovarian function of premature ovarian failure model rats induced by chemotherapy. The method of identification of human placenta derived mesenchymal stem cells via tail vein injection transplantation to premature ovarian failure rat model, the experiment was divided into HP MSCs group and stem cell culture fluid transplantation group (control group), 10 rats in each group, respectively by tail vein injection volume, injection after observation of rat ovarian structure damage. Comparison of serum sex hormone (E2, AMH, INHB) concentration. Results the rat ovarian hormone levels compared with control group, serum E2 and hpMSCs before and after transplantation INHB level had significant difference; there was no significant difference in the serum level of AMH (P=0.051); rat ovarian tissue morphology results showed that the ovarian cortex of model rats with severe injury, caudal vein after injection of hpMSCs Bcl-2 was significantly decreased. Conclusion caudal vein transplantation in hAMSCs rats improves hormone level and reduces ovarian cell apoptosis, thus repairing ovarian function.
【作者单位】：宁夏医科大学总医院;宁夏医科大学生育力保持教育部重点实验室;
【基金】：宁夏自然基金资助项目(编号:NZ14141)
【分类号】：R711.75
【正文快照】： 0 引言干细胞在体外分泌多种细胞因子,可改善卵巢微环境、甚至是全身微环境。动物实验证明,大鼠自体的脂肪间充质干细胞卵巢局部移植对化疗所致的卵巢早衰有明显的治疗作用,改善卵巢内分泌功能;羊水来源的新生儿干细胞移植后可修复卵巢功能损伤,然而新生儿胎盘间充质干细胞

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